June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Pax6 Sumoylation Is Enhanced In Cataract Lenses
Author Affiliations & Notes
  • Fangyuan Liu
    Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, Guangdong, China
  • Yunfei Liu
    Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, Guangdong, China
  • Zhong-wen Luo
    Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, Guangdong, China
  • QIAN Nie
    Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, Guangdong, China
  • Xiao-Dong Gong
    Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, Guangdong, China
  • Lili Gong
    Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, Guangdong, China
  • Lan Zhang
    Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, Guangdong, China
  • Xiangcheng Tang
    Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, Guangdong, China
  • Yizhi Liu
    Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, Guangdong, China
  • David W Li
    Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, Guangdong, China
    Truhlsen Eye Institute, University of Nebraska Medical Center, Omaha, Nebraska, United States
  • Footnotes
    Commercial Relationships   Fangyuan Liu, None; Yunfei Liu, None; Zhong-wen Luo, None; QIAN Nie, None; Xiao-Dong Gong, None; Lili Gong, None; Lan Zhang, None; Xiangcheng Tang, None; Yizhi Liu, None; David Li, None
  • Footnotes
    Support  81570824
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 3643. doi:
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    • Get Citation

      Fangyuan Liu, Yunfei Liu, Zhong-wen Luo, QIAN Nie, Xiao-Dong Gong, Lili Gong, Lan Zhang, Xiangcheng Tang, Yizhi Liu, David W Li; Pax6 Sumoylation Is Enhanced In Cataract Lenses
      . Invest. Ophthalmol. Vis. Sci. 2017;58(8):3643.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Our recent study revealed that SUMO1-mediated sumoylation is an indispensable step towards activation of p32 Pax-6, a master regulator of eye and brain development (Yan et al. 2010. PNAS). However, whether Pax6 sumoylation is altered during lens pathogenesis remains unknown. In this study, we have examined Pax6 sumoylation status from patient cataract lenses.

Methods : Immunocytochemistry and Western blot analysis were used to detect Pax6 sumoylation status in the cataract lenses from patents.

Results : Compared with the normal transparent lenses, the cataract lenses from different patients have strong 32 kd Pax6 expression. The 48 kd Pax6 is also present. In contrast, the 46 kd Pax6 is largely sumoylated, In addition, a partial 32 kd Pax6 is also sumoylated.

Conclusions : Sumoylation pattern of Pax6 is changed during lens pathogenesis. [Supported by the grant (No. 81570824) from the National Natural Science Foundation of China, Research Prevent Blindness to UNMC, and the Fundamental Funds of the State Key Laboratory of Ophthalmology in Zhongshan Ophthalmic Center, and the Graduate Scholarship from Sun Yat-sen University]

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

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