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Qian Nie, Ling Wang, Zhao-Xia Huang, David W Li; Expression of the Sumoylation Enzymes in the Ocular Tissues of Mice. Invest. Ophthalmol. Vis. Sci. 2017;58(8):3645.
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© ARVO (1962-2015); The Authors (2016-present)
The mammalian small ubiquitin-like modifiers (SUMOs) are actively involved in regulating differentiation of different cell types. In ocular tissues, sumoylation helps to determine the differentiation of cone verse rod photoreceptors. Our recent study revealed that SUMO1 and SUMO2/3 display distinct functions in regulating lens differentiation (Gong et al., 2014. PNAS). Here, we determined the expression patterns of different sumoylation enzymes in various ocular tissues
QRT-PCR, Immunocytochemistry and Western blot analysis were used for analyse the expression patterns of both sumoylation ligases and desumoylation enzymes from 5-week C57 mice.
Ligase 1 (SAE2), 2(Ubc9) and 3 (Pias1) are all strongly expressed in the cornea, retina and lens epithelial cells. Similar results are obtained for the desumoylation enzyme (SENP1). Among the 3 compartments, these sumoylation and desumoylation enzymes are much highly expressed in retina, intermediately expressed in the cornea but lower in lens epithelial cells and the lowest in lens fiber cells.
Sumoylation may play an important role in all 3 compartments of mouse eye to maintain the normal visual physiology [Supported by the grant (No. 81570824) from the National Natural Science Foundation of China, Research Prevent Blindness to UNMC, and the Fundamental Funds of the State Key Laboratory of Ophthalmology of Zhongshan Ophthalmic Center in Sun Yat-sen University]
This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.
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