June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Expression of the Sumoylation Enzymes in the Ocular Tissues of Mice
Author Affiliations & Notes
  • Qian Nie
    College of Life Sciences, Hunan Normal University, Changsha, Hunan Province, China
  • Ling Wang
    College of Life Sciences, Hunan Normal University, Changsha, Hunan Province, China
    Truhlsen Eye Institute, University of Nebraska Medical Center, Omaha, Nebraska, United States
  • Zhao-Xia Huang
    College of Life Sciences, Hunan Normal University, Changsha, Hunan Province, China
    Truhlsen Eye Institute, University of Nebraska Medical Center, Omaha, Nebraska, United States
  • David W Li
    College of Life Sciences, Hunan Normal University, Changsha, Hunan Province, China
    Truhlsen Eye Institute, University of Nebraska Medical Center, Omaha, Nebraska, United States
  • Footnotes
    Commercial Relationships   Qian Nie, None; Ling Wang, None; Zhao-Xia Huang, None; David Li, None
  • Footnotes
    Support  NSFC-81570824
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 3645. doi:
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    • Get Citation

      Qian Nie, Ling Wang, Zhao-Xia Huang, David W Li; Expression of the Sumoylation Enzymes in the Ocular Tissues of Mice. Invest. Ophthalmol. Vis. Sci. 2017;58(8):3645.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : The mammalian small ubiquitin-like modifiers (SUMOs) are actively involved in regulating differentiation of different cell types. In ocular tissues, sumoylation helps to determine the differentiation of cone verse rod photoreceptors. Our recent study revealed that SUMO1 and SUMO2/3 display distinct functions in regulating lens differentiation (Gong et al., 2014. PNAS). Here, we determined the expression patterns of different sumoylation enzymes in various ocular tissues

Methods : QRT-PCR, Immunocytochemistry and Western blot analysis were used for analyse the expression patterns of both sumoylation ligases and desumoylation enzymes from 5-week C57 mice.

Results : Ligase 1 (SAE2), 2(Ubc9) and 3 (Pias1) are all strongly expressed in the cornea, retina and lens epithelial cells. Similar results are obtained for the desumoylation enzyme (SENP1). Among the 3 compartments, these sumoylation and desumoylation enzymes are much highly expressed in retina, intermediately expressed in the cornea but lower in lens epithelial cells and the lowest in lens fiber cells.

Conclusions : Sumoylation may play an important role in all 3 compartments of mouse eye to maintain the normal visual physiology [Supported by the grant (No. 81570824) from the National Natural Science Foundation of China, Research Prevent Blindness to UNMC, and the Fundamental Funds of the State Key Laboratory of Ophthalmology of Zhongshan Ophthalmic Center in Sun Yat-sen University]

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

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