June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Conjunctival dendritic cell maturation and goblet cell density in aqueous tear deficiency
Author Affiliations & Notes
  • Stephen C Pflugfelder
    Ophthal-Ocular Surf Ctr, Baylor College of Medicine, Houston, Texas, United States
  • Fang Bian
    Ophthal-Ocular Surf Ctr, Baylor College of Medicine, Houston, Texas, United States
  • William Farley
    Ophthal-Ocular Surf Ctr, Baylor College of Medicine, Houston, Texas, United States
  • Michael E Stern
    Ophthal-Ocular Surf Ctr, Baylor College of Medicine, Houston, Texas, United States
  • Cintia S De Paiva
    Ophthal-Ocular Surf Ctr, Baylor College of Medicine, Houston, Texas, United States
  • Footnotes
    Commercial Relationships   Stephen Pflugfelder, None; Fang Bian, None; William Farley, None; Michael Stern, None; Cintia De Paiva, None
  • Footnotes
    Support  NIH Grant EY11915 (SCP), NIH Core Grants-EY002520 & EY020799, an unrestricted grant from Research to Prevent Blindness, New York, NY (SCP), the Oshman Foundation, Houston, TX (SCP), the William Stamps Farish Fund, Houston, TX (SCP), Hamill Foundation, Houston, TX (SCP), Sid W. Richardson Foundation, Ft Worth, TX (SCP)
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 3754. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to Subscribers Only
      Sign In or Create an Account ×
    • Get Citation

      Stephen C Pflugfelder, Fang Bian, William Farley, Michael E Stern, Cintia S De Paiva; Conjunctival dendritic cell maturation and goblet cell density in aqueous tear deficiency. Invest. Ophthalmol. Vis. Sci. 2017;58(8):3754.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : to test the hypothesis that clinical severity and goblet cell loss is correlated with dendritic cell (DC) maturation markers in the conjunctiva of aqueous tear deficient (ATD) patients.

Methods : Diagnosis and clinical severity grading (0-4) of ATD were based on symptom severity scores, OCT measured inferior tear meniscus height, tear break-up time and ocular surface dye staining scores. DCs were obtained from impression cytology of the superior and exposed (nasal + temporal) bulbar conjunctiva of the right eye. Goblet cell density (GCD) was measured in digital images of PAS-stained membranes taken from the temporal bulbar conjunctiva of the left eye. Flow cytometry measured percentage of cells positive for the maturation markers HLA-DR and CD86 in conjunctival DCs. Spearman correlations between GCD and DC markers were calculated.

Results : 8 control and 11 ATD subjects were evaluated. Mean clinical severity score was 2.2 (range 0-3). Compared to the control group, the percentages of CD45+HLA-DR+ and CD45+CD11c+CD86+ cells were higher in the temporal/nasal and superior (P<0.05) bulbar conjunctiva of the ATD group. Clinical severity negatively correlated with GCD (r = -0.54, P<0.05) and positively correlated with percentage of CD45+HLA-DR+ cells (r = 0.71, P<0.05). GCD was negatively correlated with percentage of CD45+HLA-DR+ cells (r = -0.61, P<0.05).

Conclusions : There is a greater percentage of mature DCs in the conjunctiva of ATD patients. DC maturation correlated with clinical severity and GC loss. It remains to be determined if DC maturation is the cause or consequence of GC loss in ATD.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×