June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Reduced micro-RNA21 expression correlates with enhanced peripheral IL-23p19 levels patients with primary Sjögren's syndrome
Author Affiliations & Notes
  • Joan Ní Gabhann
    Opthamology, Royal College of Surgeons in Ireland, Dublin, Ireland
    Opthamology, Royal Victoria Eye and Ear Hospital, Dublin, Ireland
  • Qistina Pilson
    Opthamology, Royal Victoria Eye and Ear Hospital, Dublin, Ireland
    Opthamology, Royal College of Surgeons in Ireland, Dublin, Ireland
  • Conor C Murphy
    Opthamology, Royal Victoria Eye and Ear Hospital, Dublin, Ireland
    Opthamology, Royal College of Surgeons in Ireland, Dublin, Ireland
  • Footnotes
    Commercial Relationships   Joan Ní Gabhann, None; Qistina Pilson, None; Conor C Murphy, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 3923. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to Subscribers Only
      Sign In or Create an Account ×
    • Get Citation

      Joan Ní Gabhann, Qistina Pilson, Conor C Murphy; Reduced micro-RNA21 expression correlates with enhanced peripheral IL-23p19 levels patients with primary Sjögren's syndrome. Invest. Ophthalmol. Vis. Sci. 2017;58(8):3923.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : Sjögren's syndrome (SS) is a systemic autoimmune disorder characterized by inflammation that affects mucous membranes particularly those of the exocrine glands, causing dry eyes and dry mouth. Previous investigations have suggested that dysregulated systemic inflammation contributes to the development and pathogenesis of SS. This study aimed to investigate potential mechanisms responsible for over production of pathogenic cytokines in SS patients.

Methods : Peripheral blood mononuclear cells and serum were prepared from whole blood taken from both healthy controls and pSS patients. Gene induction and micro-RNA expression were analysed by real-time PCR. Cytokine levels were determined by ELISA.

Results : We observed significantly enhanced expression of the pro-inflammatory micro-RNA, miR-155 (p≤0.05) and significantly reduced levels of the IL-10 promoting miR, miRNA-21 (p≤0.05) compared to healthy controls. In keeping with this altered pattern of miR expression we observed significantly increased serum levels of pro-inflammatory cytokines (IL-6, IL-8 and TNF-a) as well as the Th17 promoting cytokine, IL-23p19 (p ≤0.05). Altered expression of previously identified targets of miR-155 (SHIP-1, SOCS1 – potent anti-inflammatory regulators) and miR-21 (IL12p35 and PDCD4, positive regulators of inflammation) was also observed. Significantly a moderate negative correlation (r =-0.657, p≤0.05) was observed between reduced miR-21 expression and increased peripheral IL-23p19 levels in pSS patients, suggesting a link between altered miR expression and disease pathogenesis.

Conclusions : Our data suggest that abnormal expression or regulation of miRs and consequently miR regulated genes in innate immunity may contribute to the initiation and progression of SS via overproduction of pathogenic cytokines.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×