June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
MicroRNA-1 Regulates GNAQ Expression in Uveal Melanoma Cells
Author Affiliations & Notes
  • Dongsheng Yan
    School of Ophthalmology and Optometry, Wenzhou Medical University, Wenzhou, China
  • Lihua Wang
    School of Ophthalmology and Optometry, Wenzhou Medical University, Wenzhou, China
  • JIao Wang
    School of Ophthalmology and Optometry, Wenzhou Medical University, Wenzhou, China
  • Xiaoyan Chen
    School of Ophthalmology and Optometry, Wenzhou Medical University, Wenzhou, China
  • Dan-Ning Hu
    The New York Eye and Ear Infirmary, New York Medical College, New York, New York, United States
  • Footnotes
    Commercial Relationships   Dongsheng Yan, None; Lihua Wang, None; JIao Wang, None; Xiaoyan Chen, None; Dan-Ning Hu, None
  • Footnotes
    Support  National Natural Science Foundation of China (81272286)
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 3958. doi:
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    • Get Citation

      Dongsheng Yan, Lihua Wang, JIao Wang, Xiaoyan Chen, Dan-Ning Hu; MicroRNA-1 Regulates GNAQ Expression in Uveal Melanoma Cells. Invest. Ophthalmol. Vis. Sci. 2017;58(8):3958.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : GNAQ is a heterotrimeric G protein alpha subunit, which when mutated can act as an oncogene. Such a change can make it a crucial contributor to the development of uveal melanoma. There are numerous studies showing that aberrant microRNA (miRNA) expression patterns contribute to tumorigenesis. However, whether GNAQ can be modulated by miRNA, remains elusive. In a previous study, we reported that miR-1 can inhibit uveal melanoma cell proliferation and migration through the c-Met pathway. Here, we determined whether miR-1 regulates GNAQ expression in uveal melanoma cells.

Methods : Bioinformatics predicted miR-1 gene targets. Luciferase reporter assay validated putative target identity. MiR-1 or GNAQ specific siRNA was transfected into uveal melanoma cells by Lipofectamine RNAiMAX reagent. Western blot analysis was carried out to detect GNAQ expression in uveal melanoma cells. Cell proliferation and migration were measured by MTS and transwell assay, respectively.

Results : GNAQ was identified as a bona fide target of miR-1. Ectopic miR-1 downregulated GNAQ expression in uveal melanoma cells. Downregulation of GNAQ significantly inhibited uveal melanoma cell proliferation and migration.

Conclusions : Our results demonstrated that GNAQ is a miR-1 gene target in uveal melanoma cells. MiR-1 may be a promising drug target to treat uveal melanoma due to its pleiotropic regulation of multiple oncogenes.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

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