June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Outcomes of quercetin treatment on the metastatic profile of a uveal melanoma cell line
Author Affiliations & Notes
  • Laurenz L Sonnentag
    Department of Ophthalmology, University of Luebeck, Luebeck, Germany
  • Anna Kraus
    Department of Ophthalmology, University of Luebeck, Luebeck, Germany
  • Mahdy Ranjbar
    Department of Ophthalmology, University of Luebeck, Luebeck, Germany
  • Julia Lueke
    Department of Ophthalmology, University of Luebeck, Luebeck, Germany
  • Salvatore Grisanti
    Department of Ophthalmology, University of Luebeck, Luebeck, Germany
  • Aysegul Tura
    Department of Ophthalmology, University of Luebeck, Luebeck, Germany
  • Footnotes
    Commercial Relationships   Laurenz Sonnentag, None; Anna Kraus, None; Mahdy Ranjbar, None; Julia Lueke, None; Salvatore Grisanti, None; Aysegul Tura, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 3966. doi:
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    • Get Citation

      Laurenz L Sonnentag, Anna Kraus, Mahdy Ranjbar, Julia Lueke, Salvatore Grisanti, Aysegul Tura; Outcomes of quercetin treatment on the metastatic profile of a uveal melanoma cell line
      . Invest. Ophthalmol. Vis. Sci. 2017;58(8):3966.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To evaluate the efficacy of the dietary flavonoid quercetin on suppressing the viability, proliferation, and migration of the uveal melanoma cells as well as the expression of major proteins involved in metastatic activity.

Methods : Cultures of 92.1 cells were incubated with quercetin at a concentration range of 1-100 µM. Viability was analysed by the MTT-test, Live-Dead staining, and TUNEL-assay. Cell proliferation was assessed by BrdU incorporation and Ki-67 immunostaining. Migration was analyzed by the wound healing assay. Protein expression was analysed by immunocytochemistry and -blotting.

Results : Quercetin resulted in a dose-dependent reduction in cell proliferation, survival, and migration, with the effects becoming significant (P<0.05) from 25 µM onwards. Quercetin also interfered with the expression of the insulin-like growth factor-1 receptor on the cell surface, possibly by preventing the upregulation of the transcriptional coactivator YAP-1 in the nucleus.

Conclusions : These findings demonstrate the efficacy of quercetin as a novel therapy alternative for suppressing the metastatic potential of uveal melanoma cells.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

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