June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Effects of Inhibition of PDGF Family Ligands and Receptors on Pericytes and Blood Vessel Morphology in the Developing Mouse Retina
Author Affiliations & Notes
  • rajeevalochan wudali
    Ophthalmology, Regeneron Pharmaceuticals Inc., Tarrytown, New York, United States
  • Eunice Cheung
    Ophthalmology, Regeneron Pharmaceuticals Inc., Tarrytown, New York, United States
  • George Yancopoulos
    Ophthalmology, Regeneron Pharmaceuticals Inc., Tarrytown, New York, United States
  • Carl Romano
    Ophthalmology, Regeneron Pharmaceuticals Inc., Tarrytown, New York, United States
  • Ivan Lobov
    Ophthalmology, Regeneron Pharmaceuticals Inc., Tarrytown, New York, United States
  • Footnotes
    Commercial Relationships   rajeevalochan wudali, Regeneron Pharmaceuticals Inc. (E); Eunice Cheung, Regeneron Pharmaceuticals Inc. (E); George Yancopoulos, Regeneron Pharmaceuticals Inc. (E); Carl Romano, Regeneron Pharmaceuticals Inc. (E); Ivan Lobov, Regeneron Pharmaceuticals Inc. (E)
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 4056. doi:
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      rajeevalochan wudali, Eunice Cheung, George Yancopoulos, Carl Romano, Ivan Lobov; Effects of Inhibition of PDGF Family Ligands and Receptors on Pericytes and Blood Vessel Morphology in the Developing Mouse Retina
      . Invest. Ophthalmol. Vis. Sci. 2017;58(8):4056.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To determine the effects of neutralizing antibodies against PDGFRα, PDGFRβ, PDGF-B, and PDGF-D on the developing mouse retinal vasculature.

Methods : C57BL/6NTac pups were injected subcutaneously with either 3mg/kg or 25mg/kg of anti-PDGFRα, anti-PDGFRβ, anti-PDGF-B, anti-PDGF-D antibodies or control Fc protein, at post-natal day two (P2). Eyes from these pups were harvested at post-natal day five (P5). Retinas were dissected from these eyes, stained, imaged and analyzed. Pericyte coverage was assessed using anti-NG2-chondroitin sulfate proteoglycan (Millipore) and vasculature was visualized using GS Lectin I (Vector Labs).

Results : At 3mg/kg, anti-PDGFRβ treatment resulted in substantial pericyte depletion, and at 25mg/kg, almost complete pericyte depletion, abnormal blood vessel density and decreased blood vessel length. At 3mg/kg, anti-PDGF-B and anti-PDGF-D antibodies had no effect on retinal pericytes and blood vessel morphology. At a dose of 25mg/kg, anti-PDGF-B treated retinas showed partial pericyte depletion. Blocking PDGFRα had no effect on blood vessels or pericytes, at either dose.

Conclusions : Blocking PDGFRβ was highly effective at depleting pericytes from the developing retinal vasculature, while inhibiting either individual ligand for this receptor, or PDGFRα, was not.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

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