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Kazuki Nakagawa, Haruhiko Yamada, Hidetsugu Mori, Masayuki Ohnaka, kanji takahashi; Imaging of laser-induced choroidal neovascularization in mice using optical coherence tomography angiography. Invest. Ophthalmol. Vis. Sci. 2017;58(8):4074.
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© ARVO (1962-2015); The Authors (2016-present)
To evaluate the efficacy of optical coherence tomography (OCT) angiography for laser-induced choroidal neovascularization (CNV) in mice.
CNV was induced by using laser photocoagulation (GYC-2000; NIDEK Co, Gamagori, Aichi, wavelength 532nm) in the left eyes of 6 female C57BL/6J mice aged 6weeks. Laser spot with size of 80µm, pulse duration of 100ms and incident power of 200mW was applied to make rupture of Bruch’s membrane. Two eyes of the 6 eyes were irradiated by one spot, 2 eyes were irradiated by two spots with a certain distance and 2 eyes were irradiated by two spots overlapped with. Animal-dedicated adapter was installed in front of OCT and OCT angiography (RS-3000 Advance; NIDEK Co, Gamagori, Aichi). CNV images were taken at 4 or 5 days after laser photocoagulation.
In all eyes at 4 or 5 days after laser photocoagulation, laser-induced CNV was observed on OCT and OCT angiography. On OCT, CNV represented subretinal hyperreflective lesion. On OCT angiography, the figures of CNV had different types by methods of laser photocoagulation. In 2 eyes irradiated by one spot, CNV was observed as a small circular hyperflow lesion consisting of fine blood vessels, but blood vessels were not observed inner the lesion. In 2 eyes irradiated by two spots with a certain distance, CNV was observed as a hyperflow lesion in a figure of 8 consisting of thick blood vessels, and blood vessels were observed inner the lesion. In 2 eyes irradiated by two spots overlapped with, CNV was observed as a large circle-like hyperflow lesion consisting of thick vessels, and blood vessels were observed inner the lesion. On imaging of cross sectional OCT angiography in all eyes, blood signals were detected between outer layer of retina and subretinal region.
It was possible to observe laser-induced CNV in mice as a hyperflow lesion on OCT angiography. By devising methods of laser photocoagulation, CNV grows to a large size and dynamic image of CNV can be observed in detail.
This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.
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