June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Repeated subretinal surgery and removal of subretinal decaline is well tolerated – evidence from a porcine model
Author Affiliations & Notes
  • Nina Buus Sørensen
    Ophthalmology, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark
  • Kristian Klemp
    Ophthalmology, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark
  • Troels Wesenberg Kjær
    Clinical Neurophysiology, Zealand University Hospital, Roskilde, Denmark
  • Morten D De La Cour
    Ophthalmology, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark
  • Jens F Kiilgaard
    Ophthalmology, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark
  • Footnotes
    Commercial Relationships   Nina Buus Sørensen, None; Kristian Klemp, None; Troels Kjær, None; Morten De La Cour, None; Jens Kiilgaard, None
  • Footnotes
    Support  Fight for Sight Denmark, Synoptik-Fonden, Danielsen Fonden and University of Copenhagen
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 4160. doi:
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      Nina Buus Sørensen, Kristian Klemp, Troels Wesenberg Kjær, Morten D De La Cour, Jens F Kiilgaard; Repeated subretinal surgery and removal of subretinal decaline is well tolerated – evidence from a porcine model. Invest. Ophthalmol. Vis. Sci. 2017;58(8):4160.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Subretinal perfluorocarbon liquid (PFCL) is a serious complication after retinal detachment repair. It is possible to remove the PFCL surgically, but retinal damage related to the procedure is unknown. Also, increasing interest for subretinal treatment makes it relevant to examine the functional and morphological consequences of repeated subretinal manipulation. We hypothesize that PFCL in a porcine model can be injected in the subretinal space and removed with minimal effect on retinal structure and function.

Methods : Ten left eyes of healthy three-month old female domestic pigs were included. Multifocal electroretinograms (mfERGs) was recorded before surgery. Following vitrectomy a PFCL bleb (Decalin) was injected subretinally using a 41G cannula. After 14 days the Decalin was removed through a 41G cannula in combination with a 2 ml syringe and an intermediate flexible tube. Two weeks after removal a control mfERG was recorded, the pigs were enucleated and sacrificed and eyes were examined histologically. All statistics were carried out with a paired ttest in SAS Enterprise Guide 7.1® (SAS Institute Inc., Cary, NC, USA).

Results : There was no significant difference in mfERG-amplitude ratio (right/left eye) between baseline and recordings two weeks after removal of decalin (P1 (M=0.26, SD=0.80, p=0.39), 2nd order kernel (M=-0.18, SD=0.86, p=0.57), Direct Response (M=0.39, SD=0.61, p=0.12) or Induced Component (M=-0.03, SD=0.40, p=0.80)). Histologically, the photoreceptor outer segments were minimally affected. Otherwise the retina was normal 14 days after removal of decalin. In four pigs the decalin displaced inferiorly and was no longer accessible for removal.

Conclusions : Subretinal decalin can be removed within 14 days without lasting retinal damage. Decalin is a heavy liquid where the risk of displacement is high. Future studies using PFCLs to control duration of an experimental retinal separation should focus on PFCLs isodense to the vitreus body.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

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