June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Characterization of early retinal vascular changes in murine models of diabetic retinopathy: Comparison of phase-variance OCT angiography and fluorescein angiography
Author Affiliations & Notes
  • Susanna S Park
    UC Davis Eye Center/University of California, Sacramento, California, United States
  • Suman Manna
    Cell Biology and Human Anatomy, UC Davis EyePod Laboratory, Davis, California, United States
  • Pengfei Zhang
    Cell Biology and Human Anatomy, UC Davis EyePod Laboratory, Davis, California, United States
  • Jonathan Lu
    UC Davis Eye Center/University of California, Sacramento, California, United States
  • Parisa Emami-naeini
    UC Davis Eye Center/University of California, Sacramento, California, United States
  • Robert J Zawadzki
    UC Davis Eye Center/University of California, Sacramento, California, United States
    Cell Biology and Human Anatomy, UC Davis EyePod Laboratory, Davis, California, United States
  • Footnotes
    Commercial Relationships   Susanna Park, None; Suman Manna, None; Pengfei Zhang, None; Jonathan Lu, None; Parisa Emami-naeini, None; Robert Zawadzki, None
  • Footnotes
    Support  UC Davis Research in Science & Engineering (RISE) Award; NEI core (P-30 EY012576)
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 4250. doi:
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      Susanna S Park, Suman Manna, Pengfei Zhang, Jonathan Lu, Parisa Emami-naeini, Robert J Zawadzki; Characterization of early retinal vascular changes in murine models of diabetic retinopathy: Comparison of phase-variance OCT angiography and fluorescein angiography. Invest. Ophthalmol. Vis. Sci. 2017;58(8):4250.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To characterize the development of retinal vascular changes associated with diabetic retinopathy in murine models of type 1 and 2 diabetes mellitus using a multi-modal in vivo imaging system built for mouse retinal imaging.

Methods : Murine models of type 1 diabetes mellitus (i.e. streptozotocin-induced) and type 2 diabetes mellitus (i.e. C57BLKS-Leprdb homozygotes mice, db/db) were imaged serially using a multi-modal in vivo retinal imaging system built for mouse retinal imaging. This system combines a SLO fundus camera and a spectral-domain OCT instrument such that simultaneous fluorescein angiography and phase-variance OCT angiography can be performed (pv-OCTA) to evaluate retinal perfusion.

Results : Early retinal vascular changes consistent with diabetic retinopathy were noted using both fluorescein angiography and pv-OCTA in type 1 and 2 diabetic mice. Fluorescein angiography detected microaneurysmal changes in the retinal vasculature and associated vascular leakage in type 1 diabetic mice consistent with diabetic retinopathy. Although simultaneous pv-OCTA did not detect retinal vascular leakage, some of the retinal vascular microaneurysmal changes noted on fluorescein angiography also could be detected using pv-OCTA. Among db/db type 2 diabetic mice, subtle areas of probable retinal capillary non-perfusion detected on fluorescein angiography were visualized more clearly using pv-OCTA. Phase variance-OCTA allowed 3-dimensional vascular imaging, localizing the retinal vascular changes seen on fluorescein angiography images more precisely to a specific retinal layer, feature not possible using fluorescein angiography alone.

Conclusions : Simultaneous multi-modal in vivo retinal vascular imaging using fluorescein angiography and pv-OCTA allows improved detection and characterization of early retinal vascular changes associated with diabetic retinopathy in murine models of type 1 and 2 diabetes mellitus.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

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