June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Molecular Diagnostic Evaluation of Post-surgical Endophthalmitis
Author Affiliations & Notes
  • Bryan Yue
    Department of Ophthalmology, University of Washington, Seattle, Washington, United States
  • Cecilia S Lee
    Department of Ophthalmology, University of Washington, Seattle, Washington, United States
  • Sundeep Kasi
    The Retina Service of Wills Eye Hospital, MidAtlantic Retina, Wills Eye Hospital, Philadelphia, Pennsylvania, United States
  • Bryan Hong
    The Retina Service of Wills Eye Hospital, MidAtlantic Retina, Wills Eye Hospital, Philadelphia, Pennsylvania, United States
  • Lakshmi Akileswaran
    Department of Ophthalmology, University of Washington, Seattle, Washington, United States
  • Doug Baughman
    Department of Ophthalmology, University of Washington, Seattle, Washington, United States
  • Aaron Y Lee
    Department of Ophthalmology, University of Washington, Seattle, Washington, United States
  • Sunir Garg
    The Retina Service of Wills Eye Hospital, MidAtlantic Retina, Wills Eye Hospital, Philadelphia, Pennsylvania, United States
  • Russell N Van Gelder
    Department of Ophthalmology, University of Washington, Seattle, Washington, United States
  • Footnotes
    Commercial Relationships   Bryan Yue, None; Cecilia Lee, None; Sundeep Kasi, None; Bryan Hong, None; Lakshmi Akileswaran, None; Doug Baughman, None; Aaron Lee, None; Sunir Garg, Allergen (R), Allergen (F), Eyegate (F), Gentech (R), Johnson & Johnson, Centocor (F); Russell Van Gelder, None
  • Footnotes
    Support  NEI, Bethesda, MD, K23EY02392; Research to Prevent Blindness, Inc. New York, NY
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 4255. doi:
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    • Get Citation

      Bryan Yue, Cecilia S Lee, Sundeep Kasi, Bryan Hong, Lakshmi Akileswaran, Doug Baughman, Aaron Y Lee, Sunir Garg, Russell N Van Gelder; Molecular Diagnostic Evaluation of Post-surgical Endophthalmitis. Invest. Ophthalmol. Vis. Sci. 2017;58(8):4255.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To characterize the viral pathogens found in post-surgical endophthalmitis with whole genome sequencing (WGS) and polymerase chain reaction (PCR).

Methods : Patients diagnosed with endophthalmitis following intraocular procedures were recruited from Wills Eye Hospital and University of Washington from Sept 1st , 2014 to Dec 31st , 2015. All patients underwent vitreous or aqueous tap. The specimens were sent for microbial culture and additional PCR and WGS. Following DNA extraction, quantitative PCR for Torque Teno Virus (TTV), Merkel Cell Polyomavirus (MCV), and actin were performed in all samples. WGS results were analyzed by Scalable Metagenomics Alignment Research Tool (SMART). PCR results were verified via gel electrophoresis.

Results : A total of 30 samples were collected (12 culture-positive (40%), 14 culture-negative (47%), 4 unknown). WGS confirmed 100% of the bacterial culture results (12/12) and revealed substantial amounts of Staphylococcus in 2 culture-negative samples. In addition, the WGS results allowed de novo genomic reconstruction of pathogens in several samples with high specificity. Overall, 20 (66.66%) samples were positive for TTV and 15 (50%) for MCV. The presence of TTV or MCV was not associated with culture positivity by Fisher’s exact test.

Conclusions : Viral DNA sequences are unexpectedly common in endophthalmitis. The role of these viruses in this disease process remains unclear. WGS and de novo reconstruction of pathogen’s genome may become a useful technique in pathogen detection in endophthalmitis.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

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