June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Upregulation of amphiregulin, a ligand of EGFR, in healing mouse conjunctiva after incision and in cultured fibroblasts treated with TGFb1
Author Affiliations & Notes
  • Hiroki Nidegawa
    Opthalmology, Wakayama Medecal University, Wakayama, Wakayama, Japan
  • Osamu Yamanaka
    Opthalmology, Wakayama Medecal University, Wakayama, Wakayama, Japan
  • Yukihisa Takada
    Opthalmology, Wakayama Medecal University, Wakayama, Wakayama, Japan
  • Shizuya Saika
    Opthalmology, Wakayama Medecal University, Wakayama, Wakayama, Japan
  • Footnotes
    Commercial Relationships   Hiroki Nidegawa, None; Osamu Yamanaka, None; Yukihisa Takada, None; Shizuya Saika, None
  • Footnotes
    Support  none
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 4363. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to Subscribers Only
      Sign In or Create an Account ×
    • Get Citation

      Hiroki Nidegawa, Osamu Yamanaka, Yukihisa Takada, Shizuya Saika; Upregulation of amphiregulin, a ligand of EGFR, in healing mouse conjunctiva after incision and in cultured fibroblasts treated with TGFb1
      . Invest. Ophthalmol. Vis. Sci. 2017;58(8):4363.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : To examine if expression of amphiregulin in healing mouse conjunctiva after incision and if TGFb1 upregulates its expression in cultured fibroblasts. Effect of adding an EGFR inhibitor, PD168393, on fibrogenic gene expression and in vitro defect closure in the cells was also examined.

Methods : Expression pattern of amphiregulin and phospho-EGFR (pEGFR) in healing conjunctiva following circumferential incision at until day 7 by using immunohistochemistry. Effects of adding an EGFR inhibitor, PD168393, and/or TGFb1 on the closure of a liner defect produced in a confluent fibroblast culture were evaluated. Effects of adding TGFb1 on protein expression of EGFR ligands; EGF, amphiregulin, TGFα, were examined. Effects of adding TGFb1 and/or PD168393 on mRNA expression of collagen Ia1 and a-smooth muscle actin, a myofibroblast marker, were finally studied.

Results : Protein expression of amphiregulin and pEGFR was upregulated in healing conjunctiva upto until day 7. Inhibition of EGFR by PD168393 delayed defect closure of fibroblast monolayer culture. Adding TGFb1 upregulated protein expression of EGF, amphiregulin and TGFα in fibroblast culture. Myofibroblast generation by adding TGFb1 was counteracted by further addition of PD168393, while collagen Ia1 upregulation by TGFb1 was not affected by adding PD168393.

Conclusions : In vivo wounding upregulates expression of amphiregulin and phosphorylation of EGFR and the situation was mimicked in cell culture with TGFb1. Inhibition of EGFR counteracted TGFb1-induced myofibroblst generation in vitro was not affected by EGFR inhibition. Amphiregulin-EGFR system is considered to have a significant role in conjunctival wound healing.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×