June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
A novel meibum analytical method based on surface enhanced raman spectroscopy
Author Affiliations & Notes
  • Shangkun Ou
    Eye Institute of Xiamen University, Xiamen , Fujian, China
    Xiamen University affiliated Xiamen Eye Center, Xiamen, Fujian, China
  • Xin He
    Eye Institute of Xiamen University, Xiamen , Fujian, China
    Xiamen University affiliated Xiamen Eye Center, Xiamen, Fujian, China
  • Liying Zhang
    Eye Institute of Xiamen University, Xiamen , Fujian, China
    Xiamen University affiliated Xiamen Eye Center, Xiamen, Fujian, China
  • dulei zou
    Eye Institute of Xiamen University, Xiamen , Fujian, China
    Xiamen University affiliated Xiamen Eye Center, Xiamen, Fujian, China
  • Yangluowa Qu
    Eye Institute of Xiamen University, Xiamen , Fujian, China
    Xiamen University affiliated Xiamen Eye Center, Xiamen, Fujian, China
  • chengyou zuo
    Eye Institute of Xiamen University, Xiamen , Fujian, China
    Xiamen University affiliated Xiamen Eye Center, Xiamen, Fujian, China
  • Changkai Jia
    Eye Institute of Xiamen University, Xiamen , Fujian, China
    Xiamen University affiliated Xiamen Eye Center, Xiamen, Fujian, China
  • Huimin Sun
    Eye Institute of Xiamen University, Xiamen , Fujian, China
    Xiamen University affiliated Xiamen Eye Center, Xiamen, Fujian, China
  • ZUGUO LIU
    Eye Institute of Xiamen University, Xiamen , Fujian, China
    Xiamen University affiliated Xiamen Eye Center, Xiamen, Fujian, China
  • Wei Li
    Eye Institute of Xiamen University, Xiamen , Fujian, China
    Xiamen University affiliated Xiamen Eye Center, Xiamen, Fujian, China
  • Footnotes
    Commercial Relationships   Shangkun Ou, None; Xin He, None; Liying Zhang, None; dulei zou, None; Yangluowa Qu, None; chengyou zuo, None; Changkai Jia, None; Huimin Sun, None; ZUGUO LIU, None; Wei Li, None
  • Footnotes
    Support  none
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 4378. doi:
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      Shangkun Ou, Xin He, Liying Zhang, dulei zou, Yangluowa Qu, chengyou zuo, Changkai Jia, Huimin Sun, ZUGUO LIU, Wei Li; A novel meibum analytical method based on surface enhanced raman spectroscopy. Invest. Ophthalmol. Vis. Sci. 2017;58(8):4378.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : We applied the surface enhanced Raman spectroscopy (SERS) to analyze the composition of proteins, saturated fats, unsaturated fatty acids and their interaction in Meibomian gland secretion, i.e., meibum, and evaluate the pathological changes of Meibomian gland dysfunction.

Methods : Human meibum from healthy individuals and Meibomian gland dysfunction patients was expressed from eyelids and collected for direct spectroscopic study. The meibum was immediately spread onto the silicon slice coated by gold surface or preserved in glass tube. All samples were frozen under liquid nitrogen for analysis using a Raman spectrometer within two hours.

Results : Human meibum was enrich with lipid. We used the Raman band intensity ratio, I1659/I1438cm−1 and I3013/I2860cm−1, to calculate a saturation level CH2/=CH of meibum; used the Raman band intensity ratio, I1659/I1161cm−1, to calculate the relative amount of lipid and protein; and used the Raman band intensity ratio, I2860/I2934cm−1, to calculate the conformation of lipid. We found that there was significant difference (P<0.01) at I1659/I1438cm−1, I3013/I2860cm−1, and I1659/I1161cm−1 between liquid-like, granular and paste-like meibum. However, there was no significant difference (P>0.05) at I1659/I1438cm−1. Raman mapping was used for relative quantity analysis and the results showed similar as that of the Raman spectra.

Conclusions : Compared with liquid-like meibum, the unsaturated fatty acid of granular and paste-like meibum was decreased; while the protein was increased; the conformation of lipid was similar. We predict that Meibomian gland dysfunction may be accompanied by decrease of non-saturated fatty acid and increase of protein in meibum. SERS may be used as a method to analyze the meibum and determine the pathological changes of MGD.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

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