June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Short-term reproducibility of interferometry based tear film lipid layer thickness measurements in humans
Author Affiliations & Notes
  • Gerhard Garhofer
    Department of Clinical Pharmacology, Medical University of Vienna, Vienna, Austria
  • Valentin Aranha dos Santos
    Center for Medical Physics and Biomedical Engineering , Medical University of Vienna, Vienna, Austria
  • Rene Marcel Werkmeister
    Center for Medical Physics and Biomedical Engineering , Medical University of Vienna, Vienna, Austria
  • Alina Messner
    Center for Medical Physics and Biomedical Engineering , Medical University of Vienna, Vienna, Austria
  • Doreen Schmidl
    Department of Clinical Pharmacology, Medical University of Vienna, Vienna, Austria
  • Klemens Fondi
    Department of Clinical Pharmacology, Medical University of Vienna, Vienna, Austria
  • Leopold Schmetterer
    Department of Clinical Pharmacology, Medical University of Vienna, Vienna, Austria
    Center for Medical Physics and Biomedical Engineering , Medical University of Vienna, Vienna, Austria
  • Footnotes
    Commercial Relationships   Gerhard Garhofer, None; Valentin Aranha dos Santos, None; Rene Werkmeister, None; Alina Messner, None; Doreen Schmidl, None; Klemens Fondi, None; Leopold Schmetterer, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 4398. doi:
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      Gerhard Garhofer, Valentin Aranha dos Santos, Rene Marcel Werkmeister, Alina Messner, Doreen Schmidl, Klemens Fondi, Leopold Schmetterer; Short-term reproducibility of interferometry based tear film lipid layer thickness measurements in humans. Invest. Ophthalmol. Vis. Sci. 2017;58(8):4398.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Alterations in the lipid layer of the tear film play an important role in the pathophysiology of dry eye disease (DED). Recently, a new technique for the non-invasive quantitative measurement of lipid layer thickness based on interferometry has been introduced. The aim of the present study was to evaluate the short-term reproducibility of the device in healthy volunteers.

Methods : 10 subjects with no symptoms of DED participated in the study. Break up time (BUT), Schirmer I test, corneal staining and Ocular Surface Disease Index Score (OSDI) were assessed in all subjects. For measurement of lipid layer thickness, a commercially available system (LipiView Ocular Surface Interferometer; Tear Science Inc, NC, USA) was used. Lipid film thickness measurements were performed every 10 minutes for one hour to determine reproducibility of the technique. Based on these 6 consecutive measurements, the coefficient of variation was calculated as a measure of reproducibility.

Results : 3 female and 7 male subjects aged between 22 and 38 years participated in the study. Mean OSDI was 4.1±5.2, mean BUT was 10±4 seconds and mean Schirmer I test score was 17.0±7.7 mm/5sec in the study group, respectively. No corneal or conjunctival staining was present in any of the subjects. Mean lipid film thickness as measured with interferometry was 65±13nm in this healthy cohort. Based on the 6 consecutive measurements the calculated coefficient of variation of the lipid film measurement was 7.1%±3.5%.

Conclusions : : Our data shows good short-time reproducibility of the interferometry-based assessment of lipid layer thickness. Further studies on the validity of the obtained results are, however, required.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

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