June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
The effect of enhancing uptake of drug by Fusarium Solani using micelles as a vehicle
Author Affiliations & Notes
  • Junjie Zhang
    Dpt of Pharmaceutical Science, Henan Eye Institute, Henan Eye Hospital, Zhengzhou, China
  • Jingguo Li
    Dpt of Pharmaceutical Science, Henan Eye Institute, Henan Eye Hospital, Zhengzhou, China
  • Huiyun Xia
    Dpt of Pharmaceutical Science, Henan Eye Institute, Henan Eye Hospital, Zhengzhou, China
  • Tianyang Zhou
    Dpt of Pharmaceutical Science, Henan Eye Institute, Henan Eye Hospital, Zhengzhou, China
  • Susu Liu
    Henan Eye Hospital, Henan Eye Institute, Zhengzhou, China
  • Siyu He
    Henan Eye Hospital, Henan Eye Institute, Zhengzhou, China
  • Lei Han
    Henan Eye Hospital, Henan Eye Institute, Zhengzhou, China
  • Liya Wang
    Henan Eye Hospital, Henan Eye Institute, Zhengzhou, China
  • Footnotes
    Commercial Relationships   Junjie Zhang, None; Jingguo Li, None; Huiyun Xia, None; Tianyang Zhou, None; Susu Liu, None; Siyu He, None; Lei Han, None; Liya Wang, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 4443. doi:
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      Junjie Zhang, Jingguo Li, Huiyun Xia, Tianyang Zhou, Susu Liu, Siyu He, Lei Han, Liya Wang; The effect of enhancing uptake of drug by Fusarium Solani using micelles as a vehicle. Invest. Ophthalmol. Vis. Sci. 2017;58(8):4443.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose :
To study the penetration of coumarin-6 (C-6) into Fusarium solani using micelles as a vehicle.

Methods : Fungal spores were collected after the cultured on potato-dextrose agar. C-6 was used a hydrophobic model drug and was entrapped into micelles by using solvent evaporation method (C-6-M). C-6 nanoparticle was prepared by recrystallization and used as a control (C-6-N). Both C-6-M and C-6-N were diluted using saline to 0.3%. C-6-M was added into the mixture of spores suspension and sugar-salt medium (7×106 spores/ml) as an experimental group. C-6-N was added the same concentration of spores’ mixture as a control group. Both experimental and control groups were incubated under 37°C. The mixtures were taken at 0, 1, 6, 12 and 24 hours respectively, and observed under the confocal microscopy and recorded the pictures. The photos were conducted using imaris software. The fluorescent intensity was calculated in unit volume (FI/μm3). The data were compared by t-test at corresponding different time points.

Results : Fluorescence was appeared in the interior of spores or hyphae. The fluorescent intensity in the C-6-M (experimental group) was significantly higher than C-6-N (group) at different time points except at 0 and 24 h

Conclusions : Micelles as a vehicle significantly enhanced the hydrophobic model drug coumarin-6 to penetrate into the interior of Fusarium solani.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

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