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Shaomei Wang, Changqing Zhang, bin lu, Sergey Girman, Benjamin Bakondi, Joshua Saylor, Zhaohui chen; Synergistic protection from retinal degeneration by Combined stem cell therapies. Invest. Ophthalmol. Vis. Sci. 2017;58(8):4550.
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© ARVO (1962-2015); The Authors (2016-present)
Neural progenitor cells (NPCs) have been shown effective in preserving both photoreceptors and visual function after subretinal injection into rodent models for retinal degeneration. Our recent studies revealed that a subretinal injection of MSC (MSCSRI) combined with intravenous injection of MSC (MSCIV) into the Royal College of Surgeons (RCS) rats with inherited retinal degeneration offers synergistic protection from retinal degeneration and vision loss. The purpose of this study was to investigate clinical relevant NPCs combined with MSCIV as adjuvant therapy for preserving vision in the RCS rat model for retinal degeneration.
NPCSRI were performed into the RCS rats at postnatal day (P) 21-23, followed by MSCIV on the next day. Blood samples were collected at the several time points after the treatments. Visual function was examined at P60 and P90. Retinas were collected for histology and proteomic analysis.
Combined NPCSRI and MSCIV enhanced visual function as tested by optokinetic response (OKR) and electroretinography (ERG) compared with NPCSRI alone. Further examination on NPCSRI indicated the combined treatments promoted survival of NPCSRI. Proteomic analysis revealed that quality of photoreceptors, function of retina and engraftment of cells related pathway were up-regulated with MSCIV supplementary; apoptosis of neurons, generation of reactive oxygen species related pathway were down regulated.
Combined stem/progenitor treatments offer enhanced protection from retinal degeneration and vision loss. MSCIV as supporting therapy to modulate host immune response and improve overall host microenvironment may have broader application to the regenerative medicine.
This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.
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