June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Dual roles of IL-33 in ragweed-induced mouse conjunctivitis models under lacrimal gland excision.
Author Affiliations & Notes
  • Yosuke Asada
    Ophthalmology, Juntendo University School of Medicine, Tokyo, Japan
    Laboratory of Systems Biology, Center for Experimental Medicine and Systems Biology, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan
  • Susumu Nakae
    Laboratory of Systems Biology, Center for Experimental Medicine and Systems Biology, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan
  • Nobuyuki Ebihara
    Ophthalmology, Juntendo Urayasu Hospital, Chiba, Japan
  • Akira Matsuda
    Ophthalmology, Juntendo University School of Medicine, Tokyo, Japan
  • Footnotes
    Commercial Relationships   Yosuke Asada, None; Susumu Nakae, None; Nobuyuki Ebihara, None; Akira Matsuda, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 4725. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to Subscribers Only
      Sign In or Create an Account ×
    • Get Citation

      Yosuke Asada, Susumu Nakae, Nobuyuki Ebihara, Akira Matsuda; Dual roles of IL-33 in ragweed-induced mouse conjunctivitis models under lacrimal gland excision.. Invest. Ophthalmol. Vis. Sci. 2017;58(8):4725.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : We previously reported the essential roles of IL-33 in ragweed(RW)-induced experimental allergic conjunctivitis (EAC), identified existence of group 2 innate lymphoid cells (ILC2s) in lacrimal glands (LG) and effects of IL-33 with LG excision (ARVO2014, 2016). In this study, we further examined the roles of IL-33 in RW-EAC models with LG excision.

Methods : LG excision was performed unilaterally on 8-week-old female BALB/c-IL-33 deficient and BALB/c wild-type mice. The mice were sensitized with RW in alum, and then challenged 4 times with RW eye drops. Twenty minutes after the last eye-drop challenge, the EAC was evaluated using the scores for chemosis, redness, lid edema, tearing, discharge, and the number of scratching behaviors was counted for 20 minutes.
24 hours after the last challenge, the eyeballs were collected for histological analyses.

Results : Significant reduction of the numbers of scratching behaviors was observed in the RW-EAC models using IL-33 deficient mice compared to wild type mice. Histological examination revealed augmented epithelial cell defects and neutrophil predominant inflammatory cell infiltration in the ocular surface of the LG-excised RW-EAC models using IL-33 deficient mice compared to those of wild-type mice.

Conclusions : The existence of IL-33 in mouse ocular surface caused ocular irritation and induced scratching behaviors in RW-EAC models. On the other hand, IL-33 may contribute to protect eye surface from neutrophil predominant ocular surface inflammation in RW-EAC models with LG excision.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×