June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Head acceleration induced by blast wave exposure leads to abnormal visual evoked potential and eletroretinography in rats
Author Affiliations & Notes
  • Yanli Zhu
    Ocular Trauma and Vision Restoration, US Army Institute of Surgical Research, Fort Sam Houston, Texas, United States
  • Ryan Morris
    Ocular Trauma and Vision Restoration, US Army Institute of Surgical Research, Fort Sam Houston, Texas, United States
  • Peter Edsall
    Ocular Trauma and Vision Restoration, US Army Institute of Surgical Research, Fort Sam Houston, Texas, United States
  • André Akers
    Ocular Trauma and Vision Restoration, US Army Institute of Surgical Research, Fort Sam Houston, Texas, United States
  • William Elliott
    Ocular Trauma and Vision Restoration, US Army Institute of Surgical Research, Fort Sam Houston, Texas, United States
  • Brian Lund
    Ocular Trauma and Vision Restoration, US Army Institute of Surgical Research, Fort Sam Houston, Texas, United States
  • Jeffery Cleland
    Ocular Trauma and Vision Restoration, US Army Institute of Surgical Research, Fort Sam Houston, Texas, United States
  • Footnotes
    Commercial Relationships   Yanli Zhu, None; Ryan Morris, None; Peter Edsall, None; André Akers, None; William Elliott, None; Brian Lund, None; Jeffery Cleland, None
  • Footnotes
    Support  Core funded
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 4893. doi:
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      Yanli Zhu, Ryan Morris, Peter Edsall, André Akers, William Elliott, Brian Lund, Jeffery Cleland; Head acceleration induced by blast wave exposure leads to abnormal visual evoked potential and eletroretinography in rats. Invest. Ophthalmol. Vis. Sci. 2017;58(8):4893.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To characterize the changes of flash visual evoked potential (fVEP) and flash electroretinography (fERG) induced by blast exposure in rats.

Methods : Anesthetized adult male Long-Evans rats were positioned in a compressed air-driven shock tube with left side facing the incoming blast wave. In this orientation, the head underwent a significant acceleration in the horizontal plane. Rats received a single blast wave exposure with peak overpressure of 207±10kPa and a positive phase duration of 3.30±0.06ms. fVEP and fERG were recorded prior to, and at 72h and 1, 2, 4, and 8 weeks post exposure. fVEP was recorded for both eye (OU), right eye (OD) and left eye (OS) stimulations using subdermal wire electrodes. The amplitudes and latencies of waveforms of 0-N1, P1-N1 and N1-P2 were analyzed (n=5). fERG was recorded simultaneously from both eyes using contact lens electrodes. The amplitudes and latencies of a-wave and b-wave were evaluated (n=5). One way RM ANOVA, RM ANOVA on Ranks and nonparametric Signed Rank Test were used for statistical analysis.

Results : For fVEP in OU stimulation, the wave amplitude of 0-N1 were significantly decreased at 8wks post exposure (vs. the baseline p=0.027). There were no significant differences in P1-N1 and N1-P2. In OD stimulation, the wave amplitudes of 0-N1, P1-N1 and N1-P2 were significantly decreased at 8wks post exposure (vs. the baselines p=0.012, 0.046 and 0.009). In OS stimulation, there were no significant changes of wave amplitudes being observed. For fVEP latencies in OD stimulation, there were only significant differences in P1-N1 at 72h, 1w and 2wks post exposure (vs. the baseline p=0.019, 0.026 and 0.006). There were no significant differences of the latencies in OS or OU stimulations. fERG b-wave amplitudes in OD were significantly decreased at 72h and 2wks post exposure (vs. the baseline p=0.024 and 0.013). There were no significant differences of b-wave amplitude in OS, and a-wave amplitudes in both OD and OS. There were no significant changes of fERG latencies in OD and OS in both a-wave and b-wave.

Conclusions : The single blast exposure resulted in abnormal wave amplitudes and latencies in fVEP and fERG in rats. The head accelerations may cause blast related TBI and responsible for such changes. The morphological studies in visual neurons will be useful to elucidate the mechanisms of such visual dysfunction.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

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