June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Light-Induced Tearing Reflects the Spectral Characteristics of Melanopsin Phototransduction
Author Affiliations & Notes
  • Shaobo Lei
    Program in Neurosciences and Mental Health, The Hospital for Sick Children,, Toronto, Ontario, Canada
  • Xingqiao Chen
    The Krembil Research Institute, Toronto Western Hospital, Toronto, Ontario, Canada
  • Marija Zivcevska
    Program in Neurosciences and Mental Health, The Hospital for Sick Children,, Toronto, Ontario, Canada
  • Herbert C. Goltz
    Department of Ophthalmology and Vision Sciences, University of Toronto, Toronto, Ontario, Canada
    The Krembil Research Institute, Toronto Western Hospital, Toronto, Ontario, Canada
  • Agnes MF Wong
    Department of Ophthalmology and Vision Sciences, University of Toronto, Toronto, Ontario, Canada
    Department of Ophthalmology and Vision Sciences, The Hospital for Sick Children, Toronto, Ontario, Canada
  • Footnotes
    Commercial Relationships   Shaobo Lei, None; Xingqiao Chen, None; Marija Zivcevska, None; Herbert Goltz, None; Agnes Wong, None
  • Footnotes
    Support  Supported by the Canada Foundation for Innovation, John and Melinda Thompson Endowment Fund for Vision Neuroscience, and the Department of Ophthalmology and Vision Sciences at The Hospital for Sick Children.
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 5092. doi:
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      Shaobo Lei, Xingqiao Chen, Marija Zivcevska, Herbert C. Goltz, Agnes MF Wong; Light-Induced Tearing Reflects the Spectral Characteristics of Melanopsin Phototransduction. Invest. Ophthalmol. Vis. Sci. 2017;58(8):5092.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Light-induced tearing is a protective reflex associated with painful sensation of light, however, it is still unclear which light sensor provides input to this reflex. We hypothesized that light induced tearing is melanopsin-driven. This study tested this hypothesis by investigating the spectral characteristics and intensity-response function of light induced reflex tearing and its correlation with the melanopsin driven post-illumination pupil response (PIPR).

Methods : 11 visually normal participants completed the experiment, which consisted of 15 trials of 1-minute anesthetized Schirmer’s test on the right eye while pupil response was simultaneously recorded from the left eye using a video-based eye tracker. At 20 s of the trails, subjects receive either no light stimulation (baseline trial) or one flash of red (640±10 nm) or blue (467±17 nm) light stimuli of 400 ms duration presented to both eyes using a Ganzfeld stimulator. Light condition trials were presented in alternating fashion at 7 incremental steps of intensity (0.1, 1, 3.16, 10, 31.60, 100 and 400 cd/m2). PIPR was defined as the mean pupil diameter reduction from 10 to 30 s post illumination. Two-way repeated measures ANOVA and Pearson correlation coefficient were performed on tear production and PIPR data for statistical analysis.

Results : Tear production in response to 10 to 400 cd/m2 blue light was significantly greater than baseline, and it increased steadily with increasing light intensity. Red light did not induce significant tear production until 400 cd/m2. There is a positive linear correlation between PIPR and tearing in response to blue light (R= 0.61, p<0.001) but not to red light (R=0.13, p=0.25).

Conclusions : The intensity-response function and spectral characteristics of light-induced tearing are highly consistent with the features of melanopsin phototransduction. This finding is the first in-vivo evidence supporting the idea that light-induced tearing is mediated primarily by melanopsin photoactivity, which is a valuable insight into the mechanisms of photophobia.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

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