June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Drusen in the Peripheral Retina of the Alzheimer’s Eye
Author Affiliations & Notes
  • Kresimir Ukalovic
    Ophthalmology, University of British Columbia, Vancouver, British Columbia, Canada
  • Sijia Cao
    Ophthalmology, University of British Columbia, Vancouver, British Columbia, Canada
  • Sieun Lee
    Simon Fraser University, Burnaby, British Columbia, Canada
  • Qiaoyue Tang
    Ophthalmology, University of British Columbia, Vancouver, British Columbia, Canada
  • Mirza Faisal Beg
    Simon Fraser University, Burnaby, British Columbia, Canada
  • Marinko V Sarunic
    Simon Fraser University, Burnaby, British Columbia, Canada
  • Robin Hsiung
    Neurology, University of British Columbia, Vancouver, British Columbia, Canada
  • Ian R Mackenzie
    Pathology, University of British Columbia, Vancouver, British Columbia, Canada
  • Veronica Hirsch-Reinshagen
    Pathology, University of British Columbia, Vancouver, British Columbia, Canada
  • Jing Z Cui
    Ophthalmology, University of British Columbia, Vancouver, British Columbia, Canada
  • Joanne A Matsubara
    Ophthalmology, University of British Columbia, Vancouver, British Columbia, Canada
  • Footnotes
    Commercial Relationships   Kresimir Ukalovic, None; Sijia Cao, None; Sieun Lee, None; Qiaoyue Tang, None; Mirza Beg, None; Marinko Sarunic, None; Robin Hsiung, None; Ian Mackenzie, None; Veronica Hirsch-Reinshagen, None; Jing Cui, None; Joanne Matsubara, None
  • Footnotes
    Support  Brain Canada, Genome BC and VGH+UBC Foundation
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 5126. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to Subscribers Only
      Sign In or Create an Account ×
    • Get Citation

      Kresimir Ukalovic, Sijia Cao, Sieun Lee, Qiaoyue Tang, Mirza Faisal Beg, Marinko V Sarunic, Robin Hsiung, Ian R Mackenzie, Veronica Hirsch-Reinshagen, Jing Z Cui, Joanne A Matsubara; Drusen in the Peripheral Retina of the Alzheimer’s Eye. Invest. Ophthalmol. Vis. Sci. 2017;58(8):5126.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : Recent work on Alzheimer's disease (AD) diagnosis has focused on neuroimaging, in particular CT, MRI and PET; however, neuroimaging is expensive, sometimes invasive and not available to all patients. Ocular biomarkers could provide an alternative way to diagnose AD, and may be more accessible than neuroimaging. Based on previous studies suggesting that peripheral drusen may be useful as an AD biomarker, we tested the hypothesis that peripheral hard drusen would be increased in AD patients compared to controls using post-mortem eye tissues.

Methods : We assessed the histological evidence for drusen in postmortem eye tissues obtained from donors with a primary or secondary pathological diagnosis of AD. Retina from normal donors were processed and categorized into younger (≤ 55 years old) and older (>55 years old) groups. After fixation and dissection, 6-mm punches of RPE/choroid were taken in macular (centered on fovea), superior, inferior, and temporal retinal regions. In order to identify drusen, Oil Red - a stain that binds to neutral lipids - was used. Hard drusen were measured, counted and grouped into two size categories: small (≤ 63µm) and intermediate (63-125µm). The number of hard drusen was normalized to the surface area of the punch. Statistical analyses were performed using SPSS.

Results : There was a significant increase in the total number of macular hard drusen and peripheral hard drusen in older, compared to younger, normal eyes, as expected (p<0.05). However, significance was not reached for small hard drusen in AD eyes compared to older normal eyes. Interestingly, intermediate hard drusen were more commonly found in the temporal region of AD eyes compared to older normal eyes (p<0.05). Among the brain and eye tissues from AD donors, there was a strong relationship between cerebral amyloid angiopathy (CAA) score and number of intermediate hard drusen in the temporal region of the retina (R2=0.6, p<0.05).

Conclusions : Results suggest that peripheral drusen may be useful for further biomarker studies assessing the eye of AD patients, as the number of intermediate hard drusen in the temporal peripheral retina is increased compared to controls, and is positively correlated with CAA severity in corresponding AD brain tissue. Thus, non-invasive fundus imaging of temporal intermediate drusen may have biomarker potential for AD detection and/or progression and is worthy of further investigation.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×