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Talita Mizushima; Minimum inhibitory concentration of corneal storage media. Invest. Ophthalmol. Vis. Sci. 2017;58(8):5176.
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© ARVO (1962-2015); The Authors (2016-present)
The purpose was to evaluate the minimum inhibitory concentration and the minimum bactericidal concentration of corneal storage media.
Minimum inhibitory concentration (MIC) was analyzed using macrodilution method and minimum bactericidal concentration (MBC) was analyzed by microbial culture of different standard strains of Escherichia coli, Pseudomonas aeruginosa, Staphylococcus epidermidis, Staphylococcus aureus and Streptococcus pneumoniae. Samples of corneal storage media (Life40®, Optisol-GS®, Eusol- C®) were diluted for 100%, 50.00%, 25.00%, 12.50%, 6.25%, 3.12%, 1.60%, 0.80%, 0.40%, 0.20% and 0.10 %. Half milliliter of each diluted solution was placed into sterile micro tube and mixed with 0,5 mL of strain studied. After incubation, microbial growth was evaluated based on its turbidity of the corneal media. The main value considered in our study was based on the minimum concentration value of the sample without turbidity for each microorganism. And the MBC considered was the lowest concentration that demonstrates less than 99,9% of bacterial growth.
The Life40® presented the smaller MIC to Staphylococcus epidermidis 12, 5% and the smaller MBC to Pseudomonas aeruginosa 3,12%, Staphylococcus epidermidis 25% and Staphylococcus aureus 6,25%. The Optisol-GS® presented the smaller MIC to Staphylococcus epidermidis 12,5% and the smaller MBC to Staphylococcus epidermidis 25%. The Eusol- C® presented the smaller MIC to Escherichia coli 1,6%, Pseudomonas aeruginosa 0,8%, Staphylococcus aureus 1,6% and Streptococcus pneumoniae 0,40% and the smaller MBC to Escherichia coli 1,6%, Staphylococcus aureus 6,25% and Streptococcus pneumoniae 3,12%.
In vitro, the Life4C® and the Optisol-GS® presented an effective antimicrobial activity with MIC 12,5% and MBC 25%. The Eusol-C® presented an effective antimicrobial activity with MIC 25% and MBC >50%.
This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.
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