June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Effect of Recombinant Human Heat Shock Protein 27 on the Ultraviolet B-Irradiated Pterygial-derived Fibroblast
Author Affiliations & Notes
  • Yoo Jeong Park
    Ophthalmology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea (the Republic of)
  • Chan Hee Moon
    Ophthalmology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea (the Republic of)
  • Jin A Shin
    Ophthalmology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea (the Republic of)
  • Soon Suk Kang
    Ophthalmology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea (the Republic of)
  • Hungwon Tchah
    Ophthalmology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea (the Republic of)
  • Jae Yong Kim
    Ophthalmology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea (the Republic of)
  • Footnotes
    Commercial Relationships   Yoo Jeong Park, None; Chan Hee Moon, None; Jin A Shin, None; Soon Suk Kang, None; Hungwon Tchah, None; Jae Yong Kim, None
  • Footnotes
    Support  the Basic Science Research Program through the National Research Foundation of Korea (NRF) and funded by the Ministry of Education, Science, and Technology (MEST) (NRF-2010-0025662) and a grant (2016-464) from the Asan Institute for Life Sciences, Seoul, Korea.
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 5181. doi:
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      Yoo Jeong Park, Chan Hee Moon, Jin A Shin, Soon Suk Kang, Hungwon Tchah, Jae Yong Kim; Effect of Recombinant Human Heat Shock Protein 27 on the Ultraviolet B-Irradiated Pterygial-derived Fibroblast. Invest. Ophthalmol. Vis. Sci. 2017;58(8):5181.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To investigate the effect of recombinant human heat shock protein 27 (rhHSP27) on the differentiation of human pterygial fibroblast

Methods : Human conjunctival and pterygial fibroblasts were isolated and cultured from specimens of normal conjunctiva and pterygium head, which were collected during pterygium excision. Cultured conjunctival and pterygial fibroblasts were exposed to 20 mJ/cm2 of UVB irradiation with and without 4 ug/mL rhHSP27 pre-treatment. Western blot analyses of α-smooth muscle actin (α-SMA), a marker for myofibroblast, were performed in each group and quantified using Image Gauge 4.0 software. The ratio of α-SMA / GAPDH was compared

Results : Pterygial fibroblasts showed 2.85 ± 0.11 fold greater α-SMA level than normal conjunctival fibroblasts (P<0.001). When normal conjunctival fibroblasts were exposed to UVB irradiation without rhHSP27 pre-treatment, the level of α-SMA was increased by 1.92 ± 0.02 times (P<0.001). However, when normal conjunctival fibroblasts were exposed to UVB irradiation with rhHSP27 treatment, the fibroblasts demonstrated 0.82 ± 0.04 fold smaller α-SMA level than control (P=0.04)

Conclusions : The rhHSP27 had inhibitory effects on UVB irradiation-induced differentiation from normal conjunctival fibroblasts to pterygial myofibroblast. The rhHSP27 might be used as a preventive treatment for pterygium

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

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