June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
A comprehensive analysis of serum levels of prorenin/renin and inflammation-associated molecules in proliferative diabetic retinopathy
Author Affiliations & Notes
  • Keitaro Hase
    Laboratory of Ocular Cell Biology and Visual Science, Hokkaido University Graduate School of Medicine, Sapporo, Japan
    Department of Ophthalmology, Hokkaido University Graduate School of Medicine, Sapporo, Japan
  • Atsuhiro Kanda
    Laboratory of Ocular Cell Biology and Visual Science, Hokkaido University Graduate School of Medicine, Sapporo, Japan
    Department of Ophthalmology, Hokkaido University Graduate School of Medicine, Sapporo, Japan
  • Kousuke Noda
    Laboratory of Ocular Cell Biology and Visual Science, Hokkaido University Graduate School of Medicine, Sapporo, Japan
    Department of Ophthalmology, Hokkaido University Graduate School of Medicine, Sapporo, Japan
  • Susumu Ishida
    Laboratory of Ocular Cell Biology and Visual Science, Hokkaido University Graduate School of Medicine, Sapporo, Japan
    Department of Ophthalmology, Hokkaido University Graduate School of Medicine, Sapporo, Japan
  • Footnotes
    Commercial Relationships   Keitaro Hase, None; Atsuhiro Kanda, None; Kousuke Noda, None; Susumu Ishida, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 5210. doi:
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      Keitaro Hase, Atsuhiro Kanda, Kousuke Noda, Susumu Ishida; A comprehensive analysis of serum levels of prorenin/renin and inflammation-associated molecules in proliferative diabetic retinopathy. Invest. Ophthalmol. Vis. Sci. 2017;58(8):5210.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Receptor-associated prorenin system (RAPS) plays a crucial role in ocular inflammation and neovascularization, also contributing to the development of proliferative diabetic retinopathy (PDR). In this study, we explored correlations between protein levels of RAPS components including prorenin/renin and inflammation-associated molecules in the serum obtained from patients with PDR.

Methods : Serum samples were obtained from 40 consecutive patients, including 20 cases with non-angiogenic ocular diseases with idiopathic macular diseases including epiretinal membrane (ERM) and idiopathic macular hole (MH) (aged 63.4±4.3 y/o), and 20 cases with PDR (aged 62.4±8.7 y/o). Protein levels of prorenin/renin and inflammation-associated molecules, including tumor necrosis factor (TNF)-α, vascular adhesion protein-1 (VAP)-1, C-C chemokine ligand (CCL)2/monocyte chemotactic protein (MCP)-1, and interleukin (IL)-6 were determined by using a commercially available multiplex bead analysis system and enzyme-linked immunosorbent assay.

Results : As compared to control serum, protein levels of prorenin/renin, IL-1β, VAP-1, FactorD/Adipsin, and TNF-α, but not CCL2/MCP-1 or IL-6, significantly increased in PDR serum. Serum prorenin/renin correlated positively with IL-1β, FactorD/Adipsin and TNF-α in patients with PDR, but not in those with ERM and MH.

Conclusions : The current data demonstrate the elevated serum levels of prorenin/renin and correlations among prorenin/renin, IL-1β, FactorD/Adipsin and TNF-α in patients with PDR, suggesting a potential role of the RAPS in the pathogenesis of PDR.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

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