June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Non-histone nuclear protein play important roles for photoreceptor function and experimental choroidal neovascularization
Author Affiliations & Notes
  • Toshiaki Abe
    Division of Clinical Cell Therapy, Tohoku Univ School of Medicine, Sendai, Japan
  • Aya Katsuyama
    Division of Clinical Cell Therapy, Tohoku Univ School of Medicine, Sendai, Japan
  • Shinji Yamada
    Division of Clinical Cell Therapy, Tohoku Univ School of Medicine, Sendai, Japan
  • Nobuhiro Nagai
    Division of Clinical Cell Therapy, Tohoku Univ School of Medicine, Sendai, Japan
  • Reiko Daigaku
    Division of Clinical Cell Therapy, Tohoku Univ School of Medicine, Sendai, Japan
  • Kenta Ishida
    Division of Clinical Cell Therapy, Tohoku Univ School of Medicine, Sendai, Japan
  • Yuki Katsukura
    Division of Clinical Cell Therapy, Tohoku Univ School of Medicine, Sendai, Japan
  • Footnotes
    Commercial Relationships   Toshiaki Abe, Novartis Pharma K.K. (F); Aya Katsuyama, None; Shinji Yamada, None; Nobuhiro Nagai, None; Reiko Daigaku, None; Kenta Ishida, None; Yuki Katsukura, None
  • Footnotes
    Support  Grants 15ek0109073h0001 and 16ek0109073h0002 from the Japan Agency for Medical Research and Development (AMED) and Novartis Pharma K.K. (Tokyo, Japan)
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 5228. doi:
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    • Get Citation

      Toshiaki Abe, Aya Katsuyama, Shinji Yamada, Nobuhiro Nagai, Reiko Daigaku, Kenta Ishida, Yuki Katsukura; Non-histone nuclear protein play important roles for photoreceptor function and experimental choroidal neovascularization. Invest. Ophthalmol. Vis. Sci. 2017;58(8):5228.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : We reported an important role of a non-nuclear protein, high-mobility group protein N1 (HMGN1) that enhances endothelial growth factor (VEGF) in retinal pigment epithelium (RPE). We planned to examine the retinal function using genetically modified mice model and various age of normal control mice.

Methods : HMGN1 knockout mice, HMGN1tm1/tm1 was used in this study. Electroretinogram (ERG), optical coherence tomography (OCT), and histological examination (HE) as well as routine ophthalmological examinations were performed in young and old mice. These mice were also used for laser-induced choroidal neovascular (CNV) experiments and evaluated by fluorescein angiography (FA), flat mount, histological examinations and gene expressions.

Results : Statistically significant less amplitude of cone a and b waves were observed on ERG in HMGN1tm1/tm1 mice if compared to those of normal control. The tendency was clearer when we examined older HMGN1tm1/tm1 mice. HE showed significant less outer nuclear layer thickness at some points. Statistically significant less VEGF expression was observed in RPE/choroid/sclera of HMGN1tm1/tm1 when we made CNV. Statistically significant decrease of the size of laser-induced CNV was also observed in the HMGN1tm1/tm1 mice when compared to those of age-matched control mice in both FA and measurement of flat mount CNV areas.

Conclusions : HMGN1 may play important roles in some of the physiological and/or pathological conditions, such as cone function and CNV generation.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

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