June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Interface bonding with corneal crosslinking after laser in situ keratomileusis (LASIK)
Author Affiliations & Notes
  • Irene E Kochevar
    Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, Massachusetts, United States
  • Marleen Engler
    Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, Massachusetts, United States
  • Eric Beck
    Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, Massachusetts, United States
  • Theo Gunther Seiler
    Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, Massachusetts, United States
  • Footnotes
    Commercial Relationships   Irene Kochevar, Massachusetts General Hospital (P); Marleen Engler, None; Eric Beck, None; Theo Seiler, None
  • Footnotes
    Support  Swiss National Science Foundation Fellowship (Early Postdoctoral Mobility) to T.G. Seiler
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 5266. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to Subscribers Only
      Sign In or Create an Account ×
    • Get Citation

      Irene E Kochevar, Marleen Engler, Eric Beck, Theo Gunther Seiler; Interface bonding with corneal crosslinking after laser in situ keratomileusis (LASIK). Invest. Ophthalmol. Vis. Sci. 2017;58(8):5266.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose : To determine the efficacy of interface bonding with corneal crosslinking (CXL) after laser in situ keratomileusis (LASIK) ex vivo using rose bengal/green light and riboflavin/UV light.

Methods : A LASIK-flap (90 µm) was created in ex vivo rabbit eyes using a femtosecond laser. After the dissection, CXL was performed to seal the interface. In one group, interface CXL was performed using rose bengal and green-light whereas in another group CXL was performed using riboflavin and UV light. In both groups the irradiance, radiant exposure, dye concentration and imbibition time were varied to identify parameters producing strongest adhesion. In a control group only LASIK was performed. After the bonding and in the control corneas, the maximal shear force required to separate the flap from the stroma was measured.

Results : Parameters for rose bengal/green light bonding that produced a 2.2-fold increase in sheer force compared to untreated control eyes (p<0.01) were: irradiance = 180 mW/cm2, time = 14 minutes, total radiant exposure =150 J/cm2, rose bengal concentration = 0.1% and imbibition time = 2 minutes. Riboflavin/UV light parameters were 0.5% riboflavin for 2 minutes with a radiant exposure of 8.1 J/cm2 using an irradiance of 30 mW/cm2 for 4.5 minutes. These parameters produced a 2.1-fold increase compared to untreated control eyes (p<0.01).

Conclusions : Based on these ex vivo results, interface bonding during LASIK with crosslinking uisng either rose bengal or riboflavin increases the adhesion between flap and stromal bed. In vivo trials are needed to evaluate the effects of interfacial bonding over time.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×