June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Aβ-potentiated and Aβ-independent age-related changes in the lens of wild-type and Alzheimer’s Disease mice
Author Affiliations & Notes
  • Juliet A Moncaster
    Psychiatry, Boston University, Boston, Massachusetts, United States
  • Mark W Wojnarowicz
    Pathology and Laboratory Medicine, Boston University, Boston, Massachusetts, United States
    Graduate Medical Sciences, Boston University, Boston, Massachusetts, United States
  • Olga Minaeva
    Biomedical Engineering, Boston University, Boston, Massachusetts, United States
  • Srikant Sarangi
    Biomedical Engineering, Boston University, Boston, Massachusetts, United States
  • Zoe Brasher
    Graduate Medical Sciences, Boston University, Boston, Massachusetts, United States
  • Rebecca Zeng
    Graduate Medical Sciences, Boston University, Boston, Massachusetts, United States
  • Lee E Goldstein
    Psychiatry, Boston University, Boston, Massachusetts, United States
    Alzheimer's Disease Center, Boston University, Boston, Massachusetts, United States
  • Footnotes
    Commercial Relationships   Juliet Moncaster, None; Mark Wojnarowicz, None; Olga Minaeva, None; Srikant Sarangi, None; Zoe Brasher, None; Rebecca Zeng, None; Lee Goldstein, Cognoptix (P)
  • Footnotes
    Support  NASA NNX11AR05G
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 5311. doi:
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    • Get Citation

      Juliet A Moncaster, Mark W Wojnarowicz, Olga Minaeva, Srikant Sarangi, Zoe Brasher, Rebecca Zeng, Lee E Goldstein; Aβ-potentiated and Aβ-independent age-related changes in the lens of wild-type and Alzheimer’s Disease mice. Invest. Ophthalmol. Vis. Sci. 2017;58(8):5311.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Crystallins comprise ~90% of lens protein in mature lens fiber cells and undergo various post-translational modifications during aging that disrupt the normal functioning of the proteins, facilitating aggregation, insolubilization and light scattering. Crystallins have been shown to interact with Aβ in particular in Alzheimer’s disease. Here, we expand on previous work by us and others and investigated the aging effect of crystallins in an Aβ-independent and Aβ-potentiated environment and the effect on light scattering in lenses from aged (27 months) wild-type and Alzheimer’s disease transgenic mice (Tg2576).

Methods : Mice were bred, maintained and genotyped at Boston University. Mice were sacrificed at 27 months of age, perfused with phosphate buffer saline, lenses were isolated and then imaged under two different sources of light using a D70 digital Nikon camera and a custom-adapted Zeiss stereophotomicroscope.

Results : Wild-type aged mice demonstrated light scattering in two distinct concentric layers (cortical and outer nuclear) in the lens compared to young mice. Furthermore, Tg2576 aged mice showed increased scattering in the cortical layer compared to wild-type aged mice.

Conclusions : Two distinct light scattering layers of the lens are affected during aging involving Aβ-potentiated mechanisms in Tg2576 mice and Aβ-independent mechanisms in wild-type mice.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

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