June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Normal parameters of the full field ERG recorded with bipolar electrodes in Cynomolgus Macaque (Macaque fascicularis)
Author Affiliations & Notes
  • Arkady Lyubarsky
    Center for Advanced Retinal and Ophthalmic Therapeutics, SOM Univ. of Pennsylvania, Philadelphia, Pennsylvania, United States
    Vision Research Center, University of Pennsylvania, Philadelphia, Pennsylvania, United States
  • Erik Wielechowski
    Gene Therapy Program, University of Pennsylvania SOM , Philadelphia, Pennsylvania, United States
  • Tomas S Aleman
    Scheie Eye Institute, University of Pennsylvania SOM Ophthalmology, Philadelphia, Pennsylvania, United States
  • Albert M Maguire
    Center for Advanced Retinal and Ophthalmic Therapeutics, SOM Univ. of Pennsylvania, Philadelphia, Pennsylvania, United States
    Scheie Eye Institute, University of Pennsylvania SOM Ophthalmology, Philadelphia, Pennsylvania, United States
  • Gui-Shuang Ying
    Scheie Eye Institute, University of Pennsylvania SOM Ophthalmology, Philadelphia, Pennsylvania, United States
  • Erin Bote
    Gene Therapy Program, University of Pennsylvania SOM , Philadelphia, Pennsylvania, United States
  • Leah Makaron
    Gene Therapy Program, University of Pennsylvania SOM , Philadelphia, Pennsylvania, United States
  • James Wilson
    Gene Therapy Program, University of Pennsylvania SOM , Philadelphia, Pennsylvania, United States
  • Jean Bennett
    Center for Advanced Retinal and Ophthalmic Therapeutics, SOM Univ. of Pennsylvania, Philadelphia, Pennsylvania, United States
    Scheie Eye Institute, University of Pennsylvania SOM Ophthalmology, Philadelphia, Pennsylvania, United States
  • Anna P. Tretiakova
    Gene Therapy Program, University of Pennsylvania SOM , Philadelphia, Pennsylvania, United States
  • Footnotes
    Commercial Relationships   Arkady Lyubarsky, None; Erik Wielechowski, None; Tomas Aleman, None; Albert Maguire, REGENEXBIO (F), Spark Therapeutics (F); Gui-Shuang Ying, Chengdu Kanghong Biotech (C), Janssen Research & Development (C); Erin Bote, None; Leah Makaron, None; James Wilson, Dimension Therapeutics (F), Dimension Therapeutics (I), Dimension Therapeutics (C), REGENEXBIO (F), REGENEXBIO (I), REGENEXBIO (C), REGENEXBIO (P), Solid Gene Therapy (C), University of Pennsylvania, US Patent 7282199; US 8962332; US 7790449 (P); Jean Bennett, Biogen (F), Gensight Biologics (C), Gensight Biologics (R), Gensight Biologics (S), Limelight (F), REGENEXBIO (F), Sanofi (C), Spark Therapeutics (C), Spark Therapeutics (R), Spark Therapeutics (S); Anna Tretiakova, None
  • Footnotes
    Support  REGENXBIO, University of Pennsylvania Center for Advanced Retinal and Ophthalmic Therapeutics (CAROT), NIH P30 EY001583
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 5344. doi:
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      Arkady Lyubarsky, Erik Wielechowski, Tomas S Aleman, Albert M Maguire, Gui-Shuang Ying, Erin Bote, Leah Makaron, James Wilson, Jean Bennett, Anna P. Tretiakova; Normal parameters of the full field ERG recorded with bipolar electrodes in Cynomolgus Macaque (Macaque fascicularis). Invest. Ophthalmol. Vis. Sci. 2017;58(8):5344.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Cynomolgus Macaque (CM) plays an important role as an experimental subject in studies of visual abnormalities and development of potential retinal therapeutics. At the same time normal parameters of its full field electroretinograms (ERG) recorded with bipolar Burian-Allen electrodes have not been reported. We determined the parameters of full field ERG recorded in CM with bipolar Burian-Allen electrodes following the current standards of the International Society for Clinical Electrophysiology of Vision.

Methods : Twenty male and female adult CM were used in this study. Each animal/eye had a full ophthalmological exam and retinal imaging with optical coherence tomography to ensure that there were no anomalies that might impact retinal function. The ERG sessions were carried out under the dim red light conditions. Full-field stimulation was produced with a custom made Ganzfeld stimulator. A Diagnosys LLC (Lowell, MA) Espion workstation controlled the stimulator and acquired the signals. ERGs were recorded with bipolar Burian-Allen electrodes (Hansen Labs, Coralwille, IA). The intensities of flash stimuli used (cd s m-2) were: 0.01, 3 and 10 dark-adapted; 3 and 10 light-adapted.

Results : We obtained the following normal values for the ERG components (adaptation state, flash intensity, component, amplitude in mV, (standard deviation)): dark, 0.01, rod b-wave, 30 (13.1); dark, 3, a-wave, 48.9 (19.8); dark, 3, b-wave, 126 (29.5); dark, 10, a-wave, 89.4 (31.4); dark, 10, b-wave, 147.7 (34.8); light, 3, a-wave, 16.4 (4.9); light, 3, b-wave, 57.3 (16.6); light, 10, a-wave, 30 (9.8); light, 10, b-wave, 61 (17.6).

Conclusions : We determined normal values of ERG components in the CM thus establishing the baseline for other researchers interested in using ERG as a means of assessing retinal function in Macaque fascicularis.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

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