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Kiwako Mori, Toshihide Kurihara, Xiaoyan Jiang, Erisa Yotsukura, YASUHISA TANAKA, Shin-ichi Ikeda, Maki Miyauchi, Hidemasa Torii, Kazuo Tsubota; Transient exposure of high concentration oxygen induces sustained myopia in adult mice. Invest. Ophthalmol. Vis. Sci. 2017;58(8):5471.
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© ARVO (1962-2015); The Authors (2016-present)
Progression of myopia can be observed in adult human even after 20 years old, though myopia generally develops in children of school age. Particular population in adult progressive myopia may develop pathologic myopia from high myopia; however, its mechanism and therapeutics have still not been established. Several literatures reported that myopia may be induced under the circumstances of hyperbaric oxygenation in human. In this study, we examined effects of high concentration oxygen for the refraction in adult mice.
Ocular components of C57BL6/J mice including refraction, corneal radius (CR), and axial length (AL) were measured by an infrared-light refractometer (Steinbeis Transfer Center, Germany) or an optical coherence tomography device (Envisu R4310, Leica) at the postnatal 5 weeks old (P5W) as a baseline analysis. Mice were randomly divided into two groups, Oxygen group and Control group. The mice in the Oxygen group were put into a chamber with 85% oxygen concentration for 78 hours at P6W, and then put back under a normal oxygen environment. In the Control group, mice were put under normal air during the all experimental period (n=6). The ocular components were examined again 1 week and 6 weeks after oxygen exposure (at P7W and P12W, n=6). The mice were kept under a 12/12-hour light-dark cycle. Statistical analysis was performed using Student's t-test.
Compared to the Control group, the Oxygen group showed a significant myopic change at P7W and P12W. The mean values of diopter change from the baseline in each group were +10.71 vs -6.78 (at P7W, p<0.01) and +7.52 vs -7.20 (at P12W, p<0.01). The AL between the groups did not show significant difference; however, the CR (mm) in the Oxygen group was significantly short (1.50 vs 1.44 at P12W, p<0.05), and the AL/CR ratio was significantly increased (2.29 vs 2.40 at P12W, p<0.01).
Myopia was induced by high concentration oxygen exposure and sustained at least for 6 weeks in adult mice. The phenotype depends on changes in the corneal structure rather than the AL. These data suggested that high concentration oxygen may affect anterior components of the eye to induce a myopic refraction shift.
This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.
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