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Satoshi Iwamoto, Mikiko Okano, Takehiko Yokomizo, Akira Matsuda; Effect of resolvin on allergic conjunctivitis in mouse. Invest. Ophthalmol. Vis. Sci. 2017;58(8):5755.
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© ARVO (1962-2015); The Authors (2016-present)
Docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) are the ω3 poly unsaturated fatty acids that are found in fish oil. Resolvins are the metabolites of EPA and DHA. Previous reports showed that resolvins plays anti-inflammatory roles in vasculitis and asthma models in mice. In this study, we investigated the roles of resolvin D2 in allergic conjunctivitis using a mouse experimental allergic conjunctivitis model.
10-12 weeks-old female BALB/c mice were used. Mice were sensitized and challenged for 4 days by ragweed pollen. Resolvin D2 (100 ng) was injected intraperitoneally before sensitization and before each challenge. 24 hours after the last challenge, conjunctival tissue was analyzed by giemsa staining. Furthermore, serum total IgE and Th2 cytokines in the conjunctiva were quantified by Q-PCR.
The number of infiltrating eosinophil was reduced by resolvin D2 treatment. On the other hand, concentration of total IgE and expression of Th2 (Il4, Il5 and Il13) cytokines in the conjunctiva were not affected by resolvin D2 treatment.
Resolvin D2 attenuated eosinophil infiltration in a mouse allergic conjunctivitis model. The effect of resolvin is not attributed to total IgE and Th2 cytokine regulation. Resolvin might be a new therapeutic modality of allergic conjunctivitis.
This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.
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