June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Involvement of corneal lymphangiogenesis and macrophages in a murine bacterial keratitis model.
Author Affiliations & Notes
  • Akitomo Narimatsu
    Department of Ophthalmology, Tokyo Medical University, Tokyo, Japan
    Department of Microbiology, Tokyo Medical University, Tokyo, Japan
  • Takaaki Hattori
    Department of Ophthalmology, Tokyo Medical University, Tokyo, Japan
  • Naoto Koike
    Department of Microbiology, Tokyo Medical University, Tokyo, Japan
  • Kazuki Tajima
    Department of Surgery, School of Medicine, Keio University, Tokyo, Japan
  • Hayate Nakagawa
    Department of Ophthalmology, Tokyo Medical University, Tokyo, Japan
  • Haruki Katahira
    Department of Ophthalmology, Tokyo Medical University, Tokyo, Japan
  • Teruumi Minezaki
    Department of Ophthalmology, Tokyo Medical University, Tokyo, Japan
  • Shigeto Kumakura
    Department of Ophthalmology, Tokyo Medical University, Tokyo, Japan
  • Tetsuya Matsumoto
    Department of Microbiology, Tokyo Medical University, Tokyo, Japan
  • Hiroshi Goto
    Department of Ophthalmology, Tokyo Medical University, Tokyo, Japan
  • Footnotes
    Commercial Relationships   Akitomo Narimatsu, None; Takaaki Hattori, None; Naoto Koike, None; Kazuki Tajima, None; Hayate Nakagawa, None; Haruki Katahira, None; Teruumi Minezaki, None; Shigeto Kumakura, None; Tetsuya Matsumoto, None; Hiroshi Goto, None
  • Footnotes
    Support  do not use
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 5767. doi:
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    • Get Citation

      Akitomo Narimatsu, Takaaki Hattori, Naoto Koike, Kazuki Tajima, Hayate Nakagawa, Haruki Katahira, Teruumi Minezaki, Shigeto Kumakura, Tetsuya Matsumoto, Hiroshi Goto; Involvement of corneal lymphangiogenesis and macrophages in a murine bacterial keratitis model.. Invest. Ophthalmol. Vis. Sci. 2017;58(8):5767.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose :
To evaluate corneal lymphangiogenesis and involvement of tissue-resident and monocyte-derived macrophages in a bacterial keratitis model in mice using Pseudomonas aeruginosa.

Methods :
The mouse bacterial keratitis model was established using Pseudomonas aeruginosa strain PAO-1 and C57BL/6 mice. Strain PAO-1 (1×105 CFU/2.5ul) were applied in the infected group and PBS was applied in the control group after corneal epithelium was scratched. Lymphangiogenesis, angiogenesis, and macrophage infiltration were evaluated by immunostaining using whole-mount cornea at days 2, 7 and 14 post-inoculation. Anti-CD31 antibody, anti-LYVE-1 antibody, anti-CD11b antibody and anti-F4/80 antibody were used for immunostaining. Alteration of lymphangiogenesis by macrophage depletion was also evaluated intraperitoneal injection of clodronate-containing liposomes. Tissue-resident macrophages were depleted by sub-conjunctival injection and eye drop. Conversely, monocyte-derived macrophages were depleted by intraperitoneal injection.

Results :
Lymphangiogenesis and angiogenesis increased significantly in infected group at days 7 and 14 post-inoculation of PAO-1. A significant number of macrophages were observed around the lymphatic vessels in infected group. Lymphatic vessel formation was significantly reduced by monocyte-derived macrophage depletion, but not reduced by tissue-resident macrophage depletion in infected group.

Conclusions :
These results suggest that the process of lymphangiogenesis in bacterial infection of the cornea occurs at the late stage of infection presumably induced by local infiltration of monocyte-derived macrophages.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

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