June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
The effects of Escherichia coli Nissle and Salmonella typhimurium bacterial ghosts
on human conjunctival epithelial cells
Author Affiliations & Notes
  • Ehsan Ghasemian
    OCUVAC - Centre of Ocular Inflammation and Infection Laura Bassi Centre of Expertise Center of Pathophysiology, Infectiology & Immunology; Medical University of Vienna, Vienna, Austria
  • Elisabeth Stein
    OCUVAC - Centre of Ocular Inflammation and Infection Laura Bassi Centre of Expertise Center of Pathophysiology, Infectiology & Immunology; Medical University of Vienna, Vienna, Austria
  • Aleksandra Inic-Kanada
    OCUVAC - Centre of Ocular Inflammation and Infection Laura Bassi Centre of Expertise Center of Pathophysiology, Infectiology & Immunology; Medical University of Vienna, Vienna, Austria
  • Nadine Schuerer
    OCUVAC - Centre of Ocular Inflammation and Infection Laura Bassi Centre of Expertise Center of Pathophysiology, Infectiology & Immunology; Medical University of Vienna, Vienna, Austria
  • Nora Bintner
    OCUVAC - Centre of Ocular Inflammation and Infection Laura Bassi Centre of Expertise Center of Pathophysiology, Infectiology & Immunology; Medical University of Vienna, Vienna, Austria
  • Jovana Rajic
    Institute for Biological Research “Siniša Stankovic”, Department of Molecular Biology, University of Belgrade, Belgrade, Serbia
  • Talin Barisani-Asenbauer
    OCUVAC - Centre of Ocular Inflammation and Infection Laura Bassi Centre of Expertise Center of Pathophysiology, Infectiology & Immunology; Medical University of Vienna, Vienna, Austria
  • Footnotes
    Commercial Relationships   Ehsan Ghasemian, None; Elisabeth Stein, None; Aleksandra Inic-Kanada, None; Nadine Schuerer, None; Nora Bintner, None; Jovana Rajic, None; Talin Barisani-Asenbauer, None
  • Footnotes
    Support  Austrian Research Promotion Agency (FFG project number 822768).
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 5773. doi:
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      Ehsan Ghasemian, Elisabeth Stein, Aleksandra Inic-Kanada, Nadine Schuerer, Nora Bintner, Jovana Rajic, Talin Barisani-Asenbauer; The effects of Escherichia coli Nissle and Salmonella typhimurium bacterial ghosts
      on human conjunctival epithelial cells. Invest. Ophthalmol. Vis. Sci. 2017;58(8):5773.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Bacterial ghosts (BGs) are empty cell envelopes derived from Gram-negative bacteria. BGs possess all cell membrane structures, like live bacteria they are originated from, which gives a potential to use them as an adjuvant delivery system. In the present study, we investigated the potential of Escherichia coli Nissle (EcN) and Salmonella typhimurium (Sty) BGs in modulation of immune responses in human conjunctival epithelial cells (HCjE).

Methods : Confluent monolayers of HCjE cells were treated for 2 and 24 hours with EcN and Sty BGs at a concentration of 1x108 particles/ml, as well as with Interleukin- 1α as a general stimulator. 24 hours after cell stimulation the supernatants and cells were collected. Concentration of IL-6 and IL-8 were measured using ELISA. Quantification of the gene expression levels for TLR-4 and TLR-5 was performed using qRT-PCR.

Results : HCjE cells responded to EcN BGs and Sty BGs with increased secretion of pro-inflammatory cytokines and expression of toll-like receptors (TLRs). Expression of TLR-4 and TLR-5 was significantly higher in EcN BGs stimulated cells compared to Sty BGs stimulated HCjE cells (P<0.05). EcN and Sty BGs induced enhanced secretion of IL-6 and IL-8 in HCjE cells.

Conclusions : Our results suggest that both used BGs may modulate the secretion of IL-6 and IL-8 mainly via the activation of TLR-4 and TLR-5 in HCjE cells.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

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