June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
The role of LOX-1 on innate immunity against Aspergillus keratitis in mice
Author Affiliations & Notes
  • Qian Wang
    Affiliated Hospital of Qingdao University, Qingdao, Shandong, China
    University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, United States
  • Footnotes
    Commercial Relationships   Qian Wang, None
  • Footnotes
    Support   National Natural Science Foundation of China 01/01/2015-12/31/2018 The Role of LOX-1 in Innate Immunity to Aspergillus fumigatus in Corneal Epithelial Cells
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 5784. doi:
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    • Get Citation

      Qian Wang; The role of LOX-1 on innate immunity against Aspergillus keratitis in mice. Invest. Ophthalmol. Vis. Sci. 2017;58(8):5784.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : To explore the effects of lectin-like ox-LDL receptor (LOX-1) on innate immunity against Aspergillus fumigatus (A. fumigatus ) in mice cornea.

Methods : The mRNA levels of LOX-1 were tested in normal and A. fumigatus infected corneas of C57BL/6 and BALB/c mice. The expression of LOX-1, pro-inflammatory cytokines TNF- α , CXCL1 and IL-6, anti-inflammatory cytokines IL-10, and matrix metalloproteinase 9 (MMP9) were tested with treatment of LOX-1 neutralizing antibody or control IgG in A. fumigatus infected corneas of C57BL/6. Macrophages and neutrophils were extracted from susceptible C57BL/6 mice, and pretreated with LOX-1 neutralizing antibody or IgG, then stimulated with A. fumigatus. The mRNA levels of LOX-1, TNF-α, CXCL1, IL-6, IL-10 and MMP9 were evaluated by PCR.

Results : The expression of LOX-1 was significantly increased in C57BL/6 mice corneas after A. fumigatus infection compared with BABL/c mice. After treatment with LOX-1 neutralizing antibody, the expression of LOX-1, TNF-α, CXCL1, IL-6, MMP9 and IL-10 in C57BL/6 corneas were significantly decreased compared with treatment with control IgG; the expression of LOX-1, CXCL1, IL-6 and IL-10 were significantly decreased in macrophages, while TNF- α and MMP9 expressions were no difference; LOX-1, TNF-α, CXCL1, IL-6, MMP9 and IL-10 expressions were significantly decreased in neutrophils.

Conclusions : These data provided evidence that the expression of LOX-1 can affect the expression of pro-inflammatory and anti-inflammatory cytokines in fungal infected corneas, macrophages and neutrophils of C57BL/6. LOX-1 inhibition rebalanced the inflammatory response of fungal keratitis in mice.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

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