June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Analysis of Vitreous Biomarkers in Proliferative Diabetic Retinopathy and Macular Edema
Author Affiliations & Notes
  • Namrata Nandakumar
    Ophthalmology, Massachusetts Eye and Ear Infirmary/ Schepens, Brookline, Massachusetts, United States
  • Francisco J Lopez
    allergan, Irvine, California, United States
  • Gianna C Teague
    Ophthalmology, Massachusetts Eye and Ear Infirmary/ Schepens, Brookline, Massachusetts, United States
  • Kameran Lashkari
    Ophthalmology, Massachusetts Eye and Ear Infirmary/ Schepens, Brookline, Massachusetts, United States
  • Footnotes
    Commercial Relationships   Namrata Nandakumar, None; Francisco Lopez, None; Gianna Teague, None; Kameran Lashkari, None
  • Footnotes
    Support  none
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 5807. doi:
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      Namrata Nandakumar, Francisco J Lopez, Gianna C Teague, Kameran Lashkari; Analysis of Vitreous Biomarkers in Proliferative Diabetic Retinopathy and Macular Edema. Invest. Ophthalmol. Vis. Sci. 2017;58(8):5807.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : We have previously identified various pro-angiogenic and pro-inflammatory biomarkers in the vitreous of subject with proliferative diabetic retinopathy (PDR). In this study, we have performed a more detailed subgroup analysis of subject with PDR with and without diabetic macular edema (DME), cystoid macular edema (CME), and non-diabetic control.

Methods : Vitreous samples were collected from subjects with PDR (n=65), CME (n=30 ) and control (n=35) were studied by multiplex analysis (Bio-Rad Analyzer) of 31 pro-angiogenic and pro-inflammatory factors. The PDR group was further analyzed for presence (n=35) and absence of DME (n=30). Data was transformed to allow for parametric analysis including MANCOVA analysis.

Results : There were no differences in age or gender between the PDR with DME, PDR without DME, non-diabetic CME and control groups. MANCOVA analysis of 31 factors showed changes in profile of pro-angiogenic and pro-inflammatory factors
among the PDR-no DME; PDR-DME and CME groups. PLGF, VEGF-A, angiopoietin, IL-8, TNFa and leptin concentrations were higher in both PDR with or without DME (p<0.05). This pattern was distinct from the pattern observed in CME and control groups.

Conclusions : The changes in vitreous cytokines and other factors in PDR may reflect their participation in the disease process. Overall, MANCOVA analysis identified 6 distinct factors that were significantly altered in PDR with/without DME. Further studies will elucidate biomarkers specifically associated with DME.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

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