June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Daily Zeaxanthin Supplementation Improves RPE Function in a Mouse Model of RPE Oxidative Stress
Author Affiliations & Notes
  • Manas Ranjan Biswal
    Molecular Genetics and Microbiology, University of Florida College of Medicine, Gainesville, Florida, United States
  • Brad Justis
    Molecular Genetics and Microbiology, University of Florida College of Medicine, Gainesville, Florida, United States
  • Ping Yang Han
    Molecular Genetics and Microbiology, University of Florida College of Medicine, Gainesville, Florida, United States
  • Hong Li
    Molecular Genetics and Microbiology, University of Florida College of Medicine, Gainesville, Florida, United States
  • C Kathleen Dorey
    Biomedical Science, Virginia Tech Carilion School of Medicine, Roanoke, Virginia, United States
  • Dennis L Gierhart
    Research and Development, ZeaVision LLC, Chesterfield, Missouri, United States
  • Alfred S Lewin
    Molecular Genetics and Microbiology, University of Florida College of Medicine, Gainesville, Florida, United States
  • Footnotes
    Commercial Relationships   Manas Ranjan Biswal, None; Brad Justis, None; Ping Yang Han, None; Hong Li, None; C Kathleen Dorey, None; Dennis Gierhart, ZeaVision LLC (F); Alfred Lewin, None
  • Footnotes
    Support  Shaler Richardson Professorship endowment, ZeaVision LLC, National Eye Institute Grant 1K99EY027013
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 5900. doi:
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    • Get Citation

      Manas Ranjan Biswal, Brad Justis, Ping Yang Han, Hong Li, C Kathleen Dorey, Dennis L Gierhart, Alfred S Lewin; Daily Zeaxanthin Supplementation Improves RPE Function in a Mouse Model of RPE Oxidative Stress. Invest. Ophthalmol. Vis. Sci. 2017;58(8):5900.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : The purpose of the study is to test the hypothesis that zeaxanthin supplementation would prevent on oxidative damage and pathology in the retinal pigment epithelium (RPE) in Sod2flox/flox-VMD2-cre mice. RPE-specific deletion of Sod2 (manganese superoxide dismutase) in this mice model causes signs of atrophy characteristic of dry age-related macular degeneration (AMD).

Methods : Zeaxanthin beadlets were dissolved in sterile water (1gm/kg body weight). For the experimental group (n=10), diluted Zeaxanthin beadlets were delivered by daily gavage to mice starting at one month of age and extending up to five months of age. A group of mice (n=8) of same ages not receiving any supplementation served as a control. Zeaxanthin retention in the retina/RPE and liver was quantified one month following supplementation. Retinal function was evaluated by electroretinogram (ERG) and optical coherence tomography (OCT). Quantitative real time PCR of antioxidant genes was used to measure the levels of antioxidant genes after one month and four months of zeaxanthin supplementation.

Results : Zeaxanthin supplementation led to 5-fold increases in zeaxanthin in the retina/RPE (12.8ng/gram tissue) and 12-fold increases in liver (205ng/ gram tissue) compared to control mice. There was a significant decrease in a-wave and b-wave in both control and zeaxanthin-treated mice control mice by five months of age. However, we saw improved c-wave ERG amplitude (28%; p<0.01) and thicker RPE (p<0.01) in experimental mice than control mice suggesting improved function of RPE. After one month of zeaxanthin supplementation, we did not find any changes in antioxidant gene (CAT, GSTM1, HO1, NQO1, and SQSTM1) expression in the RPE/choroid, but after 4 months of supplementation there was a significant increase in expression of the same genes.

Conclusions : We conclude that daily supplementation of zeaxanthin leads to increased accumulation of this carotenoid in the retina/RPE, induction of antioxidant enzymes, and improved structure and function of the RPE.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

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