June 2017
Volume 58, Issue 8
Open Access
ARVO Annual Meeting Abstract  |   June 2017
Fucoidan Inhibits Epithelial-Mesenchymal Transition of Retinal Pigment Epithelium in Proliferative Vitreoretinopathy
Author Affiliations & Notes
  • Yao Zhang
    Department of ophthalmology, Shanghai Tenth People's Hospital, Tongji Eye Institute, Tongji University School of Medicine, Shanghai, China
  • Hui Li
    Department of ophthalmology, Shanghai Tenth People's Hospital, Tongji Eye Institute, Tongji University School of Medicine, Shanghai, China
  • Shuai Yang
    Department of ophthalmology, Shanghai Tenth People's Hospital, Tongji Eye Institute, Tongji University School of Medicine, Shanghai, China
  • Haipei Yao
    Department of ophthalmology, Shanghai Tenth People's Hospital, Tongji Eye Institute, Tongji University School of Medicine, Shanghai, China
  • Jingfa Zhang
    Department of ophthalmology, Shanghai Tenth People's Hospital, Tongji Eye Institute, Tongji University School of Medicine, Shanghai, China
  • Guo-Tong Xu
    Department of ophthalmology, Shanghai Tenth People's Hospital, Tongji Eye Institute, Tongji University School of Medicine, Shanghai, China
  • Fang Wang
    Department of ophthalmology, Shanghai Tenth People's Hospital, Tongji Eye Institute, Tongji University School of Medicine, Shanghai, China
  • Footnotes
    Commercial Relationships   Yao Zhang, None; Hui Li, None; Shuai Yang, None; Haipei Yao, None; Jingfa Zhang, None; Guo-Tong Xu, None; Fang Wang, None
  • Footnotes
    Support  This work was supported by National Natural Science Foundation of China (81300772,81271029)
Investigative Ophthalmology & Visual Science June 2017, Vol.58, 5976. doi:
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      Yao Zhang, Hui Li, Shuai Yang, Haipei Yao, Jingfa Zhang, Guo-Tong Xu, Fang Wang; Fucoidan Inhibits Epithelial-Mesenchymal Transition of Retinal Pigment Epithelium in Proliferative Vitreoretinopathy
      . Invest. Ophthalmol. Vis. Sci. 2017;58(8):5976.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose : Proliferative vitreoretinopathy (PVR) is a severe blinding complication of rhegmatogenous retinal detachment. Epithelial-mesenchymal transition (EMT) of retinal pigment epithelium (RPE) is thought to play a pivotal role in the pathogenesis of PVR. In this study, we investigated the potential effect of Fucoidan on EMT in PVR animal model as well as in cultured human RPE cells line ARPE-19.

Methods : Pigmented rabbits and ARPE-19 cells were employed in this study. PVR animal model was induced in pigmented rabbits on Day 1 with intravitreal injection of primary rabbit RPE cells immediately after removal of 0.1ml of aqueous humor. Fucoidan was administered intravitreally on Day 1 and Day 7; the animal with intravitreal PBS injection served as control. Two weeks later, the fundus was examined.TGF-β1-treated ARPE-19 cells were incubated with or without Fucoidan; 48 hours later, the cells were harvested and the expressions of Fibronectin, α-SMA, E-cadherin and ZO-1 were studied with immunoblotting.

Results : PVR progression was suppressed significantly by Fucoidan compared to that injected with PBS. In vitro, TGF-β1-induced the decrease of E-cadherin and the increase of α-SMA and fibronectin could be reversed by Fucoidan.

Conclusions : The preliminary data showed Fucoidan could ameliorate PVR development via inhibiting EMT of RPE. The detailed mechanisms need further exploration.

This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017.

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