%0 Journal Article
%A Rakoczy, Elizabeth P
%A Lai, Chooi-May
%A Magno, Aaron
%A Wikstrom, Matthew
%A French, Martyn
%A Schwartz, Steven D
%A Blumenkranz, Mark S
%A Chalberg, Thomas Walter
%A Degli-Espoti, Mariapia
%A Constable, Ian J
%T One-year analysis of sFlt-1 and VEGF proteins and immunological markers in the bodily fluids of subjects participating in the Phase I rAAV.sFlt-1 trial.
%B Investigative Ophthalmology & Visual Science
%D 2015
%J Investigative Ophthalmology & Visual Science
%V 56
%N 7 
%P 1281-1281
%@ 1552-5783
%X  To assess whether subretinal injection of rAAV.sFlt-1 influences sFlt-1 and VEGF levels in the tears, saliva, blood, or urine of subjects participating in the Phase 1 trial up to one year post-treatment. A total of 8 subjects with wet AMD were enrolled, including 2 control subjects and 6 subjects treated with rAAV.sFlt-1 (3 low dose: 10E10 vg and 3 high dose: 10E11 vg) via subretinal injection. Assays for sFlt-1 and VEGF proteins in serum, urine and saliva were conducted at regular intervals. ELISAs were also performed at the same time points to detect both total anti-AAV antibodies and neutralizing antibodies in serum. The systemic immune response to rAAV.sFlt-1 was also monitored at these intervals via ELISpot on peripheral blood mononuclear cells. sFlt-1 levels averaged 53 pg/ml in serum (range 0-432 pg/ml), 44 pg/ml in urine (range 0-438), and 364 pg/ml in saliva (range 0-2338 pg/ml). There was no correlation in sFlt-1 levels within subjects among different fluids measured. There was no elevation of sFlt-1 levels observed in serum, saliva, blood, or urine following subretinal injection of rAAV.sFlt-1. In rAAV.sFlt-1 treated subjects, VEGF levels averaged 312 pg/ml in serum at baseline (169-443pg/ml) and 247pg/ml (range 73-546 pg/ml) at 1 year (p<0.4684). At baseline, three subjects (1 treated and 2 controls) tested positive for neutralizing antibodies against AAV2. Neutralizing antibodies remained unchanged in 5/6 subjects and increased in one subject at Week 3. Anti-AAV T-cell immunity was measured using IFN-g ELISpot, which remained unchanged in 5/6 subjects and one subject showed transient elevation. There was no correlation between either neutralizing antibody titres or ELISpot and clinical outcome. Subretinal injection of rAAV.sFLT-1 was highly reproducible. Most adverse events were mild, none were related to gene therapy. There was no evidence of retinal atrophy. Following rAAV.sFLT-1 therapy there was no statistically significant change in sFLT-1 or VEGF levels up to 1 year post treatment.These results suggest that subretinal injection of rAAV.sFlt-1 does not alter VEGF and sFlt-1 protein levels outside of the eye and that the immune response is limited and not related to any clinical observations. These result support further research of gene therapy as a potential treatment for wet AMD. 
%[ 5/31/2020