RT Journal Article A1 Paquet-Durand, Francois A1 Beck, Susanne A1 Michalakis, Stylianos A1 Goldmann, Tobias A1 Huber, Gesine A1 Mühlfriedel, Regine A1 Becirovic, Elvir A1 Wolfrum, Uwe A1 Tanimoto, Naoyuki A1 Seeliger, Mathias T1 Genetic Ablation Of Rod Cyclic-nucleotide Gated (cng) Channels Rescues Rd1 Mouse Retinal Degeneration And Preserves Cone Photoreceptor Function JF Investigative Ophthalmology & Visual Science JO Invest. Ophthalmol. Vis. Sci. YR 2011 VO 52 IS 14 SP 1806 OP 1806 SN 1552-5783 AB Purpose: The rd1 mouse is one of the most commonly studied animal models for retinitis pigmentosa (RP), a severe and frequently blinding human retinal degeneration. In several decades of research, the link between mutation-induced increase in photoreceptor cGMP levels and the extremely rapid cell death has remained poorly understood. Here, we set out to study the role of cyclic-nucleotide-gated (CNG) channels in the degenerative process, by crossing CNGb1-/- animals with rd1 mutant mice. Methods: Single mutant rd1 and CNGb1 animals as well as double mutant CNGb1-/- x rd1 animals were analysed using in vivo optic coherence tomography and scanning laser ophthalmoscopy imaging techniques, combined with electroretinographic functional testing. Ex vivo analysis included assessment of rod and cone photoreceptor markers, degenerative markers (calpain activity, TUNEL), cGMP contents, and electron microscopic ultrastructural analysis of rod outer segments. Results: In Cngb1-/- x rd1 double mutants devoid of regular CNG channels, cGMP levels were strongly elevated, but rod photoreceptor viability and outer segment morphology were greatly improved. Importantly, cone photoreceptors, the basis for high-resolution daylight and colour vision, survived and remained functional for extended periods of time. Conclusions: We demonstrated the major importance of CNG channels for rod photoreceptor loss supporting the hypothesis of deleterious calcium (Ca2+)-influx as a cause of rapid rod cell death. Furthermore, our results suggest that targeting rod CNG channels, rather than general Ca2+-channel blockade, is a promising symptomatic approach to treat otherwise incurable forms of cGMP-related RP. RD 1/19/2021