RT Journal Article
A1 Liu, Wenzhong
A1 Wang, Shoujian
A1 Soetikno, Brian
A1 Yi, Ji
A1 Zhang, Kevin
A1 Chen, Siyu
A1 Linsenmeier, Robert A.
A1 Sorenson, Christine M.
A1 Sheibani, Nader
A1 Zhang, Hao F.
T1 Increased Retinal Oxygen Metabolism Precedes Microvascular Alterations in Type 1 Diabetic Mice
JF Investigative Ophthalmology & Visual Science
JO Invest. Ophthalmol. Vis. Sci.
YR 2017
DO 10.1167/iovs.16-20600
VO 58
IS 2
SP 981
OP 989
SN 1552-5783
AB    To investigate inner retinal oxygen metabolic rate (IRMRO2) during early stages of type 1 diabetes in a transgenic mouse model.    In current study, we involved seven diabetic mice (Akita/+, TSP1−/−) and seven control mice (TSP1−/−), and applied visible-light optical coherence tomography (vis-OCT) to image functional parameters including retinal blood flow rate, oxygen saturation (sO2) and the IRMRO2 value longitudinally from 5 weeks of age to 13 weeks of age. After imaging at 13 weeks of age, we analyzed the imaging results, and examined histology of mouse retina.    Between diabetic mice and the control group, we observed significant differences in venous sO2 from 9 weeks of age (P = 0.006), and significant increment in IRMRO2 from 11 weeks of age (P = 0.001) in diabetic mice compared with control group. We did not find significant differences in retinal blood flow rate as well as arterial sO2 during imaging between diabetic and control mice. Histologic examination of diabetic and control mice at 13 weeks of age also revealed no anatomical retinal alternations.    In diabetic retinopathy, complications in retinal oxygen metabolism may occur before changes of retinal anatomical structure.  
RD 3/5/2021
UL https://doi.org/10.1167/iovs.16-20600