RT Journal Article
A1 Wu, Kun-Chao
A1 Xiang, Lue
A1 Chen, Xue-Jiao
A1 Chen, Xue-Wen
A1 Zhou, Gao-Hui
A1 Zhang,  Bo-Wen 
A1 Jin, Zi-Bing
T1 Targeted deletion of miR-182 leads to photoreceptor dysfunction in mice
JF Investigative Ophthalmology & Visual Science
JO Invest. Ophthalmol. Vis. Sci.
YR 2017
VO 58
IS 8
SP 352
OP 352
SN 1552-5783
AB    MiR-182 is the most abundantly retinal miRNA in mammals. Although the deletion of miR-182 reveals none apparently structural abnormalities in the retina, the role of miR-182 in the retinal function remains unclear yet. In this study, we aimed to determine whether ablation of miR-182 alters photoreceptor function based on the miR-182 knock out (KO) mice we generated previously.    The color fundus camera and high resolution spectral-domain optical coherence tomography (SD-OCT) were utilized to screen the morphological and structural changes for the fundus and retinal layers of miR-182 KO mice. Ganzfeld electroretinagram (ERG) was carried out to examine the retinal function. Quantitatively compensational expression of other two members of miR-183 clusters (miR-96 and miR-183) within retina of the miR-182 KO mice was determined using qRT-PCR. Potential target genes of miR-182 were computated and verified though the bioinformatics and RNAseq. Two-tailed student's t-test was used for statistical analysis.    We did not observe retinal structural change in color fundus photograph (FP) and SD-OCT imaging as well as compensational expression of miR-96 and miR-183 in the KO mice. Scotopic ERG showed that both a-wave and b-wave amplitude were decreased. In addition, maximal and photopic ERG were abnormal. Furthermore, we found up-regulated different expression genes in the retina of miR-182 mice.    In summary, we for the first time determined the retinal functional defect in the miR-182 KO mice. Our finding suggested that the miR-182 maybe play a critical role in maintaining the functionality of photoreceptors in mice.  This is an abstract that was submitted for the 2017 ARVO Annual Meeting, held in Baltimore, MD, May 7-11, 2017. 
RD 1/20/2021