March 1971
Volume 10, Issue 3
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Articles  |   March 1971
Peroxidase Diffusion in the Normal and Photocoagulated Retina
Author Affiliations
  • GHOLAM A. PEYMAN
    Department of Ophthalmology and the Jules Stein Eye Institute, UCLA School of Medicine, Los Angeles, Calif.
  • MANFRED SPITZNAS
    Department of Ophthalmology and the Jules Stein Eye Institute, UCLA School of Medicine, Los Angeles, Calif.
  • BRADLEY R. STRAATSMA
    Department of Ophthalmology and the Jules Stein Eye Institute, UCLA School of Medicine, Los Angeles, Calif.
Investigative Ophthalmology & Visual Science March 1971, Vol.10, 181-189. doi:
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      GHOLAM A. PEYMAN, MANFRED SPITZNAS, BRADLEY R. STRAATSMA; Peroxidase Diffusion in the Normal and Photocoagulated Retina. Invest. Ophthalmol. Vis. Sci. 1971;10(3):181-189.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

To study diffusion pathioays in normal and photocoagulated mammalian retina, xenon photocoagulation was applied to one eye in each of a group of rabbits; three iveeks later, peroxidase was injected in both eyes; thereafter, the animals were killed at intervals and the tissues were studied with electron microscopy. In the normal eyes, peroxidase diffused rapidly through the intercellular spaces in all layers of the sensory retina, but diffusion stopped abruptly at the zonulae occludentes in the intercellular spaces of the pigment epithelium. Three weeks after photocoagulation, peroxidase diffused rapidly through all layers of the scar to come in contact with Bruch's membrane. Consideration has been given to the anatomical basis for these observations, and to the mechanism of the action of photocoagulation in certain retinal diseases.

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