November 1972
Volume 11, Issue 11
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Articles  |   November 1972
Calcium-Induced Aggregation of Bovine Lens Alpha Crystallins
Author Affiliations
  • J. A. JEDZINIAK
    The Department of Physics, Massachusetts Institute of Technology, Cambridge, Mass.
  • J. H. KINOSHITA
    The Laboratory of Vision Research, National Eye Institute, National Institute of Health, Bethesda, Md.
  • E. M. YATES
    The Department of Physics, Massachusetts Institute of Technology, Cambridge, Mass.
  • L. O. HOCKER
    The Department of Physics, Massachusetts Institute of Technology, Cambridge, Mass.
  • G. B. BENEDEK
    The Department of Physics, Massachusetts Institute of Technology, Cambridge, Mass.
Investigative Ophthalmology & Visual Science November 1972, Vol.11, 905-915. doi:
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      J. A. JEDZINIAK, J. H. KINOSHITA, E. M. YATES, L. O. HOCKER, G. B. BENEDEK; Calcium-Induced Aggregation of Bovine Lens Alpha Crystallins. Invest. Ophthalmol. Vis. Sci. 1972;11(11):905-915.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

The theory of light scattering indicates that aggregation of the crystalline lens proteins can produce opacity. Furthermore, it is known that calcium increases in the cataractous lens. We have, therefore, investigated the effectiveness of calcium ion in producmg aggregates of the soluble proteins of the bovine lens. The experimental results show: (1) CaCl2 produce's aggregates of the soluble protein of the lens; (2) the pre-α and at fraction of the lens protein are the specific targets of calcium ion even in the presence of sulfhydryl reducing reagent; (3) the aggregation can be reversed and transparency restored when calcium is removed either by dialysis or chelation; and (4) the molecular weight distribution and index of refraction of the aggregates have been measured.

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