August 1972
Volume 11, Issue 8
Free
Articles  |   August 1972
Effect of Lysosomes on Corneal Endothelium: An in Vivo Study
Author Affiliations & Notes
  • DHARMENDRA V. ARYA
    Estelle Doheny Eye Foundation, Los Angeles, Calif.
  • JEAN MANNAGH
    Estelle Doheny Eye Foundation, Los Angeles, Calif.
  • A. RAY IRVINE, Jr.
    Estelle Doheny Eye Foundation, Los Angeles, Calif.
  • ROBERT E. LEVINE
    Estelle Doheny Eye Foundation, Los Angeles, Calif.
  • ZOLTON YUHASZ
    Estelle Doheny Eye Foundation, Los Angeles, Calif.
  • Reprint requests to Dharmendra V. Arya, Ph.D., 272 South Lake Street, Los Angeles, California 90057. 
Investigative Ophthalmology & Visual Science August 1972, Vol.11, 655-661. doi:
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      DHARMENDRA V. ARYA, JEAN MANNAGH, A. RAY IRVINE, ROBERT E. LEVINE, ZOLTON YUHASZ; Effect of Lysosomes on Corneal Endothelium: An in Vivo Study. Invest. Ophthalmol. Vis. Sci. 1972;11(8):655-661.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Rabbit peritoneal polymorphonuclear leukocyte lysosomes were injected into the anterior chamber. The cornea was studiedin paraffin cross-sections. To study the corneal endothelium, fiat preparations were made. Nitroblue tetrazolium staining was used to study endothelial cell viability. The mitotic activity of the endothelium was estimated by tritiated thymidine incorporation. The corneas of lysosome-injected eyes were edematous and their endothelium showed cellular pleomorphism and metachromatism. Increased mitotic activity, indicating reparative response secondary to the celldamage, was observed in the endothelium of lysosome- injected eyes.

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