January 1974
Volume 13, Issue 1
Free
Articles  |   January 1974
Human Corneal Cells in Vitro: Morphology and Histocompatibility (HL-A) Antigens of Pure Cell Populations
Author Affiliations
  • DAVID A. NEWSOME
    Laboratory of Vision Research, National Institutes of Health, United States Department of Health, Education, and Welfare, Bethesda, Md. 20014
  • MITSUO TAKASUGI
    Department of Surgery, School of Medicine, University of California, Los Angeles, Calif. 90024
  • KENNETH R. KENYON
    Laboratory of Vision Research, National Institutes of Health, United States Department of Health, Education, and Welfare, Bethesda, Md. 20014
  • WALTER F. STARK
    Clinical Branch, National Eye Institute, National Institutes of Health, United States Department of Health, Education, and Welfare, Bethesda, Md. 20014
  • GERHARD OPELZ
    National Institute of Child Health and Human Development, National Institutes of Health, United States Department of Health, Education, and Welfare, Bethesda, Md. 20014
Investigative Ophthalmology & Visual Science January 1974, Vol.13, 23-32. doi:
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      DAVID A. NEWSOME, MITSUO TAKASUGI, KENNETH R. KENYON, WALTER F. STARK, GERHARD OPELZ; Human Corneal Cells in Vitro: Morphology and Histocompatibility (HL-A) Antigens of Pure Cell Populations. Invest. Ophthalmol. Vis. Sci. 1974;13(1):23-32.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Pure populations of human comeal epithelial cells, keratocytes, and endothelial cells were established in vitro. Morphologic examination of the cells by phase contrast and electron microscopy showed that, even after multiple serial passages, the cells retained structural specializations typical of their tissues of origin. Using a modified cytotoxic plating inhibition test, HL-A histocompatibility antigens could successfully be detected on all cultured cells. The results of HL-A typing were generally concordant between corneal cells and lymphocytes from the same donor. This study provides direct evidence that HL-A antigens are present on cultured corneal cells, and suggests that such cells may prove useful in the study of immunologic graft rejection encountered in keratoplasty.

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