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DIANE L. VAN-HORN, RICHARD O. SCHULTZ; Endothelial Survival in Cryopreserved Human Corneas: A Scanning Electron Microscopic Study. Invest. Ophthalmol. Vis. Sci. 1974;13(1):7-16.
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The endothelium of human corneas, cryopreserved by the method of Kaufman and Capella, was examined by scanning and transmission electron microscopy to determine how many cells survive the freezing and thawing procedures. The entire endothelial layer was present in all specimens and most cells were structurally intact unless the time between death of the donor and freezing of the cornea was prolonged. Storage at -196° C. for longer than one year did not appear to have an adverse effect on the endothelium.
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