January 1974
Volume 13, Issue 1
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Articles  |   January 1974
Endothelial Survival in Cryopreserved Human Corneas: A Scanning Electron Microscopic Study
Author Affiliations
  • DIANE L. VAN-HORN
    Departments of Ophthalmology and Physiology, The Medical College of Wisconsin, Milwaukee, and the Research Service, Veterans Administration Center Wood (Milwaukee), Wise.
  • RICHARD O. SCHULTZ
    Departments of Ophthalmology and Physiology, The Medical College of Wisconsin, Milwaukee, and the Research Service, Veterans Administration Center Wood (Milwaukee), Wise.
Investigative Ophthalmology & Visual Science January 1974, Vol.13, 7-16. doi:
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      DIANE L. VAN-HORN, RICHARD O. SCHULTZ; Endothelial Survival in Cryopreserved Human Corneas: A Scanning Electron Microscopic Study. Invest. Ophthalmol. Vis. Sci. 1974;13(1):7-16.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

The endothelium of human corneas, cryopreserved by the method of Kaufman and Capella, was examined by scanning and transmission electron microscopy to determine how many cells survive the freezing and thawing procedures. The entire endothelial layer was present in all specimens and most cells were structurally intact unless the time between death of the donor and freezing of the cornea was prolonged. Storage at -196° C. for longer than one year did not appear to have an adverse effect on the endothelium.

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