December 1974
Volume 13, Issue 12
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Articles  |   December 1974
Cell-Surface Receptors Of Normal, Regenerating, And Cultured Corneal Epithelial And Endothelial Cells
Author Affiliations
  • W. MANSKI
    Departments of Microbiology and Ophthalmology, College of Physicians and Surgeons, Columbia University New York, N. Y.
  • T. L. WHITESIDE
    Departments of Microbiology and Ophthalmology, College of Physicians and Surgeons, Columbia University New York, N. Y.
Investigative Ophthalmology & Visual Science December 1974, Vol.13, 935-944. doi:
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      W. MANSKI, T. L. WHITESIDE; Cell-Surface Receptors Of Normal, Regenerating, And Cultured Corneal Epithelial And Endothelial Cells. Invest. Ophthalmol. Vis. Sci. 1974;13(12):935-944.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

The presence of cell-surface receptors accessible to antibodies on regenerating and cultured, but not on resting or metabolically inhibited, corneal epithelial and endothelial cells was established by immunofluorescence or immunocytotoxicity. The metabolically dependent antigens (MDA) can be demonstrated in the corneal cells (membranes) even when absent from the surface of viable cells. This is evidenced by the ability of normal cells to induce an antibody response and by absorption experiments. Thus, comeal regeneration as well as corneal cell culture apparently involves an unmasking of the MDA. In contrast to the restricted specificity of the MDA, the ubiquitous Forssman or blood-group antigens on chicken corneal cells reacted with antibodies independently of their metabolic state. The immunocytotoxic .reactions involving Forssman cell-surface antigens were enhanced by complement, whereas, similar reactions involving the MDA were complement independent.

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