February 1963
Volume 2, Issue 1
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Articles  |   February 1963
The Use of Tetrazolium to Distinguish Between Retinal Receptors Exposed and Not Exposed to Light
Author Affiliations
  • JAY M. ENOCH
    Department of Ophthalmology, Washington University Medical School and The Oscar Johnson Institute, St. Louis, Mo.
Investigative Ophthalmology & Visual Science February 1963, Vol.2, 16-23. doi:https://doi.org/
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      JAY M. ENOCH; The Use of Tetrazolium to Distinguish Between Retinal Receptors Exposed and Not Exposed to Light. Invest. Ophthalmol. Vis. Sci. 1963;2(1):16-23. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Using a histochemical technique, employing nitro blue tetrazolium added to a tissue culture medium, with succinate as substrate, it has been possible to stain selectively the ellipsoids of retinal receptors which have been exposed to light. The comparable portions of dark-adapted receptors did not stain. Albino rats, albino rabbits, and squirrel monkeys have been tested. Similar results xoere obtained whether the retina was exposed to light in vivo or in vitro. The effect was present in rods and cones.

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