August 1964
Volume 3, Issue 4
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Articles  |   August 1964
Study of Lens Metabolism in vivo
Author Affiliations
  • GEORGE E. GARCIA
    Howe Laboratory of Ophthalmology, Harvard University Medical School, Massachusetts Eye and Ear Infirmary, Boston, Mass.
  • THOMAS M. AABERG
    Howe Laboratory of Ophthalmology, Harvard University Medical School, Massachusetts Eye and Ear Infirmary, Boston, Mass.
  • CARL KUPFER
    Howe Laboratory of Ophthalmology, Harvard University Medical School, Massachusetts Eye and Ear Infirmary, Boston, Mass.
Investigative Ophthalmology & Visual Science August 1964, Vol.3, 384-387. doi:https://doi.org/
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      GEORGE E. GARCIA, THOMAS M. AABERG, CARL KUPFER; Study of Lens Metabolism in vivo . Invest. Ophthalmol. Vis. Sci. 1964;3(4):384-387. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

A technique for studying lens metabolism by anterior chamber perfusion in vivo is presented. A particular advantage of this technique is that the lens epithelium delineated by the pupillary space is the only portion of the lens surface exposed to the perfusate, there by providing in vivo isolation of this area. The uptake of P-32-labeled inorganic phosphate, Na-24, and Rb-86 by the lens to as investigated. The uptake of Rb-86 teas by far the greatest, while the uptake of P-32 and Na-24 was quantitatively similar despite the differences in their concentration gradients. The distribution of the isotopes in the lens, as determined by autoradiography, is also presented.

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