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Sandra Bacman, Alejandro Berra, Leonor Sterin-Borda, Enri Borda; Muscarinic Acetylcholine Receptor Antibodies as a New Marker of Dry Eye Sjögren Syndrome. Invest. Ophthalmol. Vis. Sci. 2001;42(2):321-327.
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purpose. The authors investigated whether circulating autoantibodies against
M3 muscarinic acetylcholine receptors (mAChRs) could be a
new marker for diagnosis for primary and secondary Sjögren
syndrome (SS) dry eye.
methods. Enzyme-linked immunosorbent assay (ELISA) using both rat exorbital
lacrimal gland acinar cell membranes and synthetic 25-mer peptide as
antigens was used to determine autoantibodies against acinar cells and
M3 mAChRs. Also, nitric oxide synthase (NOS) activity was
assessed to determine the biological effect of these autoantibodies in
relation to the M3 mAChR.
results. Sera from dry eye primary SS (pSS) or secondary SS (sSS) patients
tested by ELISA recognized membrane lacrimal gland acinar cells
antigens and the synthetic 25-mer peptide, corresponding to the second
extracellular loop of human M3 mAChRs. Moreover, the IgG
fraction and the corresponding affinity-purified anti-M3 peptide autoantibodies from the same patients were able to activate NOS
coupled to lacrimal gland M3 mAChRs. As controls, IgG and
sera from women without dry eye with or without rheumatoid arthritis
and from normal control subjects gave negative results on ELISA and
biological assay; thus demonstrating the specificity of the reaction.
conclusions. Autoantibodies against mAChR may be considered among the serum factors
implicated in the pathophysiology of the development of pSS dry eyes
and could be a new marker to differentiate SS dry eyes from non-SS dry
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