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Jane C. Sowden, James K. L. Holt, Moritz Meins, Hazel K. Smith, Shomi S. Bhattacharya; Expression of Drosophila omb-Related T-Box Genes in the Developing Human and Mouse Neural Retina. Invest. Ophthalmol. Vis. Sci. 2001;42(13):3095-3102.
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purpose. To examine the role of Drosophila optomotor blind
(omb)–related T-box genes in development of human and
methods. Mouse Tbx2, Tbx3, and Tbx5 and human TBX2 cDNAs were isolated from retinal cDNA
libraries by hybridization to the Drosophila omb gene.
Gene expression patterns in developing retina were analyzed by in situ
results. TBX2/Tbx2, TBX3/Tbx3, and TBX5/Tbx5 were expressed asymmetrically across the
embryonic neural retina with highest levels of mRNA within dorsal and
peripheral retina. The dorsoventral gradient of TBX2 expression disappeared before the ganglion cell layer (GCL) formed. Its
expression then became restricted to the inner neuroblastic retina and
later to the GCL and inner nuclear layer (INL). The dorsal expression
domains of TBX5/Tbx5 and TBX3/Tbx3 were
maintained during formation of the GCL. As the retina matured, TBX3/Tbx3 expression was restricted to the INL, and TBX5/Tbx5 was expressed within the GCL.
conclusions. The expression pattern of TBX2, TBX3, and TBX5 within the developing retina supports the idea that
the encoded transcription factors play a role in providing positional
information important for topographic mapping and in differentiation of
distinct cell types across the laminar axis of the
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