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Virginia L. Calder, Boaz Shaer, Mohammed Muhaya, Marie Mclauchlan, Russell V. Pearson, Gilles Jolly, Hamish M. A. Towler, Susan Lightman; Increased CD4+ Expression and Decreased IL-10 in the Anterior Chamber in Idiopathic Uveitis. Invest. Ophthalmol. Vis. Sci. 1999;40(9):2019-2024.
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purpose. To compare cell types and cytokines in aqueous humor from patients with
uveitis either occurring in association with a systemic disease or
apparently isolated and not associated with a systemic disease.
methods. Cells were collected by centrifugation of fresh aqueous humor from
uveitis and controls, and immunofluorescence techniques were performed
with markers for T cells, B cells, and monocytes. Cytokines were
measured in the aqueous supernatants, and serum samples were assayed
for soluble interleukin-2 receptors.
results. When aqueous samples from idiopathic uveitis were compared with those
from uveitis associated with a systemic disease, there were increases
in CD3+, CD4+ (p =
0.001), and activated CD4+ T cells (p =
0.02) and a decrease in B cells (p = 0.0013). This
was not reflected in the peripheral blood where there were no
differences in the cell types or in soluble interleukin-2 receptor
levels. No cells were obtainable from control aqueous. Interleukins-10
and -12, interferon-γ, and transforming growth factor-β2 were
detected in aqueous supernatants. Interleukin-10 was reduced
(p = 0.024) in uveitis in comparison with controls.
conclusions. The results suggest a selective recruitment of CD4+ T cells
within aqueous humor but only in idiopathic uveitis. In both disease
groups there was a decrease in the immunoregulatory cytokine
interleukin-10, which might enable an immune response to occur in an
otherwise highly immunosuppressive microenvironment. Increases in
activated CD4+ T cells combined with depressed
interleukin-10 levels could partially explain why, for example, in
acute anterior uveitis, the inflammatory disease is often more
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